Cytokine-regulated secretion of nerve growth factor from cultured rat neonatal astrocytes
Cytokine-regulated secretion of nerve growth factor from cultured rat neonatal astrocytes
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DOI：
10.1007/s004240000020
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发表时间：
2000-01-01
影响因子：
4.5
通讯作者：
Carman-Krzan, M
中科院分区：
文献类型：
--
作者：
Juric, DM;Carman-Krzan, M
参与炎症细胞因子调节神经生长因子（NGF）释放的相互作用：白细胞介素-1 β（IL-1 β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β 1将培养的星形胶质细胞暴露于IL-1 β，IL-6，而TGF-β 1则刺激神经生长因子的分泌。与对照细胞相比，用IL-1 β（10 U/ml）、IL-6（5 ng/ml）和TGF-β 1（5 ng/ml）处理细胞24小时导致NGF分泌增加3倍、1.8倍和2.8倍。相反，TNF-α（30 ng/ml）本身对NGF释放没有刺激作用，而与IL-1 β和TNF-α共刺激星形胶质细胞显示出协同相互作用。用IL-1 β和TGF-β 1处理星形胶质细胞以相加的方式增加NGF分泌，而同时应用IL-1 β和IL-6导致对NGF分泌的抑制作用。我们的研究结果表明，细胞因子之间的相互作用，导致通过不同的调节机制刺激神经生长因子的分泌。
Interactions involved in the regulation of nerve growth factor (NGF) release by inflammatory cytokines: interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta 1 (TGF-beta 1) were examined in rat neonatal cortical astrocytes in primary culture.Exposure of cultured astrocytes to IL-1 beta, IL-6, and TGF-beta 1 resulted in the stimulation of NGF secretion. Treatment of cells for 24 h with IL-1 beta (10 U/ml), IL-6 (5 ng/ml) and TGF-beta 1 (5 ng/ml) caused 3-, 1.8-, and 2.8-fold increase in NGF secretion as compared to the control cells. In contrast TNF-alpha (30 ng/ml) by itself had no stimulatory action on NGF release whereas co-stimulation of astrocytes with IL-1 beta and TNF-alpha showed a synergistic interaction. Go-treatment of astrocytes with IL-1 beta and TGF-beta 1 increased NGF secretion in an additive way, whereas simultaneous application of IL-1 beta and IL-6 resulted in the inhibitory effect on NGF secretion. Our results suggest that interactions between cytokines used cause the stimulation of NGF secretion through different regulatory mechanisms.