RELATIVE CONTRIBUTIONS OF THIOLTRANSFERASE-DEPENDENT AND THIOREDOXIN-DEPENDENT SYSTEMS IN REDUCTION OF LOW-MOLECULAR-MASS AND PROTEIN DISULFIDES
RELATIVE CONTRIBUTIONS OF THIOLTRANSFERASE-DEPENDENT AND THIOREDOXIN-DEPENDENT SYSTEMS IN REDUCTION OF LOW-MOLECULAR-MASS AND PROTEIN DISULFIDES
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DOI：
10.1042/bj2130519
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发表时间：
1983-01-01
影响因子：
4.1
通讯作者：
HOLMGREN, A
中科院分区：
文献类型：
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作者：
MANNERVIK, B;AXELSSON, K;HOLMGREN, A
存在两种能够还原低分子量化合物以及多肽和蛋白质中的二硫键的酶系统。一种由硫醇转移酶与还原型谷胱甘肽和谷胱甘肽还原酶组合组成，第二种由硫氧还蛋白与硫氧还蛋白还原酶组合组成。本研究评估了催化大鼠肝细胞质中各种底物二硫键还原的相对有效性。硫醇转移酶依赖性系统在减少小分子方面更有效。硫氧还蛋白系统最有效地减少胰岛素。牛胰蛋白酶是硫醇转移酶的更好底物，部分蛋白水解的牛血清白蛋白对于这两个系统同样有效。因此，在蛋白质二硫键的情况下，所用特定底物的性质决定了两个还原系统中哪一个更重要。
Two enzyme systems capable of reducing disulfide bonds both in low-MW compounds and in polypeptides and proteins exist. One consists of thioltransferase in combination with reduced glutathione and glutathione reductase, and the second consists of thioredoxin in combination with thioredoxin reductase. The relative effectiveness in catalyzing disulfide reduction of various substrates in rat liver cytosol was evaluated in the present study. The thioltransferase-dependent system was more efficient in reducing small molecules. Insulin was most effectively reduced by the thioredoxin system. Bovine trypsin was a better substrate for thioltransferase, and partially proteolyzed bovine serum albumin was equally good for the 2 systems. Thus, in the case of protein disulfide bonds, the nature of the particular substrate used determines which of the 2 reducing systems is the more important.