The genetic interaction between HIV and the antibody repertoire
The genetic interaction between HIV and the antibody repertoire
复制标题
HIV 和抗体库之间的遗传相互作用
DOI：
10.1101/646968
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ryan D. Hernandez
中科院分区：
文献类型：
--
作者：
N. Strauli;Emily Fryer;Olivia Pham;M. Abdel;S. Facente;C. Pilcher;P. Pennings;S. Pillai;Ryan D. Hernandez
人类免疫缺陷病毒（human immunodeficiency virus，HIV）与抗体库（antibody repertoire，AbR）在慢性感染过程中的相互作用可以为HIV疫苗研究提供重要信息，但尚未在系统水平上得到很好的表征。我们对10名艾滋病毒感染者、抗逆转录病毒（ART）初治者的HIV群体和AbR进行了深度测序，每个人有10-20个纵向样本，时间跨度为4-14年。我们的无偏测序方法鉴定了显示与自体HIV群体相互作用的证据的AbR分区。我们发现，这些HIV相关的分区富集已知的HIV广泛中和抗体（bnAb）的V基因片段，这表明可以通过时间序列遗传数据来识别AbR的HIV应答组分。尽管有证据表明在亚群水平上存在较大规模的AbR/HIV相互作用，但我们几乎没有发现共同进化的证据。这表明，共同进化要么是罕见的，要么是难以检测的，这对疫苗设计具有重要意义。
The interaction between human immunodeficiency virus (HIV) and the antibody repertoire (AbR) during chronic infection can provide important information for HIV vaccine research, yet has not been well-characterized on a systems level. We deeply sequenced the HIV population and the AbR of ten HIV-infected, antiretroviral (ART)-naïve individuals, each with 10-20 longitudinal samples spanning 4-14 years. Our unbiased sequencing approach identified partitions of AbRs showing evidence of interaction with autologous HIV populations. We show that these HIV-associated partitions are enriched for the V gene segments of known HIV broadly neutralizing antibodies (bnAbs), indicating that the HIV-responding component of the AbR can be identified via time-series genetic data. Despite this evidence for larger-scale AbR/HIV interactions at the sub-population level, we found little to no evidence for coevolution. This suggests that coevolution is either rare, or hard to detect, which has important vaccine design implications.
影响因子：
6.7
作者：
Henn MR;Boutwell CL;Charlebois P;Lennon NJ;Power KA;Macalalad AR;Berlin AM;Malboeuf CM;Ryan EM;Gnerre S;Zody MC;Erlich RL;Green LM;Berical A;Wang Y;Casali M;Streeck H;Bloom AK;Dudek T;Tully D;Newman R;Axten KL;Gladden AD;Battis L;Kemper M;Zeng Q;Shea TP;Gujja S;Zedlack C;Gasser O;Brander C;Hess C;Günthard HF;Brumme ZL;Brumme CJ;Bazner S;Rychert J;Tinsley JP;Mayer KH;Rosenberg E;Pereyra F;Levin JZ;Young SK;Jessen H;Altfeld M;Birren BW;Walker BD;Allen TM
通讯作者：
Allen TM
影响因子：
32.4
作者：
MacLeod, Daniel T.;Choi, Nancy M.;Briney, Bryan;Garces, Fernando;Ver, Lorena S.;Landais, Elise;Murrell, Ben;Wrin, Terri;Kilembe, William;Liang, Chi-Hui;Ramos, Alejandra;Bian, Chaoran B.;Wickramasinghe, Lalinda;Kong, Leopold;Eren, Kemal;Wu, Chung-Yi;Wong, Chi-Huey;Pond, Sergei L. Kosakovsky;Wilson, Ian A.;Burton, Dennis R.;Poignard, Pascal
通讯作者：
Poignard, Pascal