Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice.
Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice.
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DOI：
10.1039/c4fo00525b
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发表时间：
2015-01
期刊：
影响因子：
6.1
通讯作者：
Tianshun Zhang;Y. Yamashita;M. Yasuda;Norio Yamamoto;H. Ashida
中科院分区：
文献类型：
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作者：
Tianshun Zhang;Y. Yamashita;M. Yasuda;Norio Yamamoto;H. Ashida
明日叶（亚洲的食品成分和民间药物）中的两种主要查耳酮：4-羟基德里素和黄当归醇已被证明可以调节 3T3-L1 和 HepG2 细胞的脂质代谢。在这项研究中，我们研究了明日叶提取物对高脂（HF）饮食小鼠肥胖的影响及其基于脂肪组织和肝脏脂质代谢的潜在机制。 C57BL/6 小鼠喂食正常或添加明日叶提取物（0.01% 和 0.1%，w/w）的 HF 饮食 16 周。明日叶提取物抑制高频饮食引起的体重增加和白色脂肪组织中的脂肪沉积，降低血浆胆固醇、葡萄糖和胰岛素水平，增加脂联素水平，降低甘油三酯和肝脏胆固醇含量，增加脂肪组织和肝脏中AMP激活蛋白激酶（AMPK）的磷酸化，抑制脂肪生成 通过下调过氧化物酶体增殖物激活受体（PPAR）γ、CCAAT/增强子结合蛋白α和甾醇调节元件结合蛋白1（SREBP1）的表达，抑制脂肪组织；通过下调SREBP1及其靶酶脂肪酸合成酶的表达，抑制肝脏中的脂肪生成；通过上调脂肪酸合成酶的表达，促进脂肪酸氧化。 肉碱棕榈酰转移酶-1A 和 PPARα。总之，明日叶提取物可能通过脂肪组织和肝脏中 AMPK 的磷酸化来调节脂质代谢，从而预防肥胖。
Two main chalcones, 4-hydroxyderricin and xanthoangelol, from Ashitaba, which is a food ingredient and a folk medicine in Asia, have been demonstrated to modulate lipid metabolism in 3T3-L1 and HepG2 cells. In this study, we investigated the effects of Ashitaba extract on adiposity in mice fed a high-fat (HF) diet and its underlying mechanisms based on adipose tissue and hepatic lipid metabolism. C57BL/6 mice were fed a normal or HF diet supplemented with Ashitaba extract (0.01% and 0.1%, w/w) for 16 weeks. Ashitaba extract suppressed the HF diet-induced body weight gain and fat deposition in white adipose tissue, reduced plasma cholesterol, glucose, and insulin levels, increased the adiponectin level, lowered triglyceride and the liver cholesterol content, increased phosphorylation of AMP-activated protein kinase (AMPK) in adipose tissue and liver, inhibited lipogenesis in adipose tissue by down-expression of peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer-binding protein α and sterol regulatory element-binding protein 1 (SREBP1), inhibited lipogenesis in the liver by down-expression of SREBP1 and its target enzyme fatty acid synthase, and promoted fatty acid oxidation by up-expression of carnitine palmitoyltransferase-1A and PPARα. In conclusion, Ashitaba extract can possibly prevent adiposity through modulating lipid metabolism through phosphorylation of AMPK in adipose tissue and liver.