AphA is required for biofilm formation, motility, and virulence in pandemic Vibrio parahaemolyticus
AphA is required for biofilm formation, motility, and virulence in pandemic Vibrio parahaemolyticus
复制标题
AphA 是大流行性副溶血弧菌生物膜形成、运动和毒力所必需的。
DOI：
10.1016/j.ijfoodmicro.2012.11.004
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发表时间：
2013-01-01
影响因子：
5.4
通讯作者：
Zhou, Dongsheng
中科院分区：
文献类型：
--
作者：
Wang, Li;Ling, Yan;Zhou, Dongsheng
AphA是弧菌中的一个小的PadR家族DNA结合调节因子。AphA已被证明参与转录自抑制、小鼠肠道定植和致死、生物膜形成和霍乱弧菌的群体感应。副溶血性弧菌的AphA蛋白与霍乱弧菌的AphA蛋白具有85%的同一性，具有相同数量的氨基酸。在这项工作中，aphA无效突变体构建从野生型流行株的副溶血性弧菌的表型变化的表征。AphA是副溶血性弧菌中生物膜形成所需的，并且相对于野生型亲本菌株，aphA突变体中生物膜胞外多糖基质的产生减少是其生物膜形成减少的原因。AphA也是副溶血性弧菌的最佳游泳和群集运动所必需的。此外，AphA是小鼠致死性和细胞毒活性所必需的，但aphA缺失对肠毒性没有影响。(c)2012爱思唯尔有限公司版权所有。
AphA is a small PadR-family DNA-binding regulator in vibrios. AphA has been shown to be involved in transcriptional auto-repression, intestinal colonization and lethality in mice, biofilm formation, and quorum sensing in Vibrio cholerae. The AphA protein of Vibrio parahaemolyticus has 85% identity to that of V. cholerae with the same number of amino acids. In this work, the aphA null mutant was constructed from a wild-type pandemic strain of V. parahaemolyticus for characterization of the phenotypic changes. AphA is required for biofilm formation in V. parahaemolyticus, and a decreased production of biofilm exopolysaccharide matrix in the aphA mutant relative to the wild-type parent strain accounts for its reduced biofilm formation. AphA is also necessary for the optimal swimming and swarming motility of V. parahaemolyticus. In addition, AphA is essential for lethality in mice and cytotoxic activity, but the aphA deletion did not have effect on enterotoxicity. (c) 2012 Elsevier B.V. All rights reserved.