A novel cell type responsible for marrow graft rejection in mice. T cells with NK phenotype cause acute rejection of marrow grafts.
A novel cell type responsible for marrow graft rejection in mice. T cells with NK phenotype cause acute rejection of marrow grafts.
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DOI：
10.4049/jimmunol.142.10.3423
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发表时间：
1989-05
影响因子：
4.4
通讯作者：
B. Yankelevich;C. Knobloch;M. Nowicki;G. Dennert
中科院分区：
文献类型：
--
作者：
B. Yankelevich;C. Knobloch;M. Nowicki;G. Dennert
同种异体和半同种异体骨髓移植物的急性排斥反应长期以来被认为是天然免疫系统的功能，因为它与小鼠中的NK活性具有许多共同特征。使用最近开发的体内过继转移试验，其中脾细胞从能够排斥特定骨髓移植物的小鼠转移到不能这样做的小鼠中，我们表明，负责诱导骨髓移植物排斥反应的细胞确实显示NK细胞的表型：它们缺乏T细胞Ag CD 4和CD 8，但表达NK Ag NK 1和ASGM 1。过继转移细胞引起的排斥反应是非常特异的--这一特征表明转移细胞有一个特异的识别过程。为了阐明这些细胞上的识别结构，我们发现它们表达CD 3，并且很可能表达TCR的β链。具有NK 1+、CD 3+、CD 4-、CD 8-表型的高度纯化的应答细胞在转移到无应答受体中时引起特异性骨髓移植排斥。我们的结论是骨髓移植物的急性排斥反应是由一个细胞，表达NK表型，但T细胞谱系。这可能表明急性骨髓移植排斥反应的特异性是由涉及TCR的特异性识别过程引起的。
Acute rejection of allogeneic and semiallogeneic marrow grafts has long been considered to be a function of the natural immune system because it shares many features with NK activity in mice. With the use of a recently developed in vivo adoptive transfer assay in which spleen cells are transferred from mice able to reject a particular marrow graft into mice that fail to do so, we show that the cells responsible for induction of marrow graft rejection indeed display the phenotype of NK cells: they lack the T cell Ag CD4 and CD8 but express the NK Ag NK1 and ASGM1. The rejection induced by adoptively transferred cells is exquisitely specific--a feature that points to a specific recognition process by the transferred cells. To elucidate what the recognition structure on these cells may be we found that they express CD3 and most likely the beta-chain of the TCR. Highly purified responder cells with the NK1+, CD3+, CD4-, CD8- phenotype, when transferred into nonresponder recipients, cause specific marrow graft rejection. We conclude that the acute rejection of bone marrow grafts is caused by a cell that expresses NK phenotype but is of T cell lineage. This may suggest the specificity of acute marrow graft rejection is caused by a specific recognition process that involves TCR.