Surface structure of in vitro assembled bacteriophage lambda polyheads.
Surface structure of in vitro assembled bacteriophage lambda polyheads.
复制标题
体外组装的噬菌体 lambda 多头的表面结构。
DOI：
10.1016/0022-2836(76)90065-6
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发表时间：
1976
影响因子：
5.6
通讯作者：
T. Hohn
中科院分区：
文献类型：
--
作者：
M. Wurtz;J. Kistler;T. Hohn
通过衣壳蛋白的自组装可以获得管状异常噬菌体λ衣壳(Polyhead)。从表面结构上可以区分出三种类型的噬菌体，它们分别对应于小λ、扩大的小λ(D−-Head)和噬菌体头部。后一种多头由两种不同的蛋白质(pE和pd)组成，它们排列在复合的六角晶格中，另两种蛋白质(Pe)排列在六角晶格中。每种类型都有自己的晶格常数。阴性染色和冷冻干燥-阴影后均观察到多聚体，并用光学滤光法对数据进行分析。模型被用来解释多头及其球形类似物的表面精细结构，以及噬菌体λ头成熟过程中的构象变化。
Tubular aberrant phage λ capsoids (polyheads) can be obtained by self-assembly of capsid protein. Three types of polyheads can be distinguished which correspond in their surface structure to petit λ, enlarged petit λ (D−-head) and phage head. The latter type of polyhead consists of two different proteins (pEand pD) arranged in a composite hexagonal lattice, the other two of one type of protein (pE) arranged in hexagonal lattices. Each type has its own lattice constant. Polyheads were observed both after negative staining and freeze drying-shadowing and data were exploited by optical filitration methods. Models are proposed to elucidate the surface fine structure of polyheads and their spherical analogues, and the conformational changes occurring during maturation of the phage λ head.