Mitochondrial NAD dependent aldehyde dehydrogenase either from yeast or human replaces yeast cytoplasmic NADP dependent aldehyde dehydrogenase for the aerobic growth of yeast on ethanol.
Mitochondrial NAD dependent aldehyde dehydrogenase either from yeast or human replaces yeast cytoplasmic NADP dependent aldehyde dehydrogenase for the aerobic growth of yeast on ethanol.
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来自酵母或人的线粒体 NAD 依赖性乙醛脱氢酶取代了酵母细胞质 NADP 依赖性乙醛脱氢酶，用于酵母在乙醇上的需氧生长。
DOI：
10.1016/j.bbagen.2013.02.010
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Weiner,Henry
中科院分区：
文献类型：
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作者：
Mukhopadhyay,Abhijit;Wei,Baoxian;Weiner,Henry
背景在先前的研究中，我们从酿酒酵母中删除了三个参与乙醇代谢的乙醛脱氢酶（ALDH）基因，发现三重缺失的酵母菌株不能以乙醇作为唯一碳源生长。ALDH为NADP依赖的胞浆ALDH 1、NAD依赖的线粒体ALDH 2和NAD/NADP依赖的线粒体ALDH 5。双缺失菌株Δ ALDH 2 +Δ ALDH 5或Δ ALDH 1 +Δ ALDH 5能在乙醇中生长。双缺失菌株Δ ALDH 1 +Δ ALDH 2不能在乙醇中生长。将来自酵母或人的线粒体NAD依赖性ALDH置于酵母胞质溶胶中。结果在本研究中，我们发现活性非常低的细胞质ALDH 1突变形式几乎不支持三重缺失菌株（Δ ALDH 1 +Δ ALDH 2 +Δ ALDH 5）在乙醇上的生长。发现NADP依赖性ALDH 1对菌株在乙醇上生长的重要性，我们检查了如果将线粒体形式置于胞质溶胶中，来自酵母或人的NAD依赖性线粒体ALDH 2是否能够支持三重缺失菌株在乙醇上的生长。我们发现，NAD依赖的线粒体ALDH 2从酵母或人类是活跃的胞质溶胶和支持的三重删除菌株的生长上ethanol. CONCLUSION这项研究表明，辅酶的ALDH偏好是不关键的酵母细胞质溶胶中的生长上ethanol.GENERAL SIGNIFICANCE本研究提供了一个基础，以了解辅酶的ALDH偏好在酵母的乙醇代谢。
BACKGROUNDIn a previous study, we deleted three aldehyde dehydrogenase (ALDH) genes, involved in ethanol metabolism, from yeast Saccharomyces cerevisiae and found that the triple deleted yeast strain did not grow on ethanol as sole carbon source. The ALDHs were NADP dependent cytosolic ALDH1, NAD dependent mitochondrial ALDH2 and NAD/NADP dependent mitochondrial ALDH5. Double deleted strain ΔALDH2+ΔALDH5 or ΔALDH1+ΔALDH5 could grow on ethanol. However, the double deleted strain ΔALDH1+ΔALDH2 did not grow in ethanol.METHODSTriple deleted yeast strain was used. Mitochondrial NAD dependent ALDH from yeast or human was placed in yeast cytosol.RESULTSIn the present study we found that a mutant form of cytoplasmic ALDH1 with very low activity barely supported the growth of the triple deleted strain (ΔALDH1+ΔALDH2+ΔALDH5) on ethanol. Finding the importance of NADP dependent ALDH1 on the growth of the strain on ethanol we examined if NAD dependent mitochondrial ALDH2 either from yeast or human would be able to support the growth of the triple deleted strain on ethanol if the mitochondrial form was placed in cytosol. We found that the NAD dependent mitochondrial ALDH2 from yeast or human was active in cytosol and supported the growth of the triple deleted strain on ethanol.CONCLUSIONThis study showed that coenzyme preference of ALDH is not critical in cytosol of yeast for the growth on ethanol.GENERAL SIGNIFICANCEThe present study provides a basis to understand the coenzyme preference of ALDH in ethanol metabolism in yeast.