SYNTHESIS AND SECRETION OF PROTEINS RESEMBLING PLATELET-DERIVED GROWTH-FACTOR BY HUMAN GLIOBLASTOMA AND FIBRO-SARCOMA CELLS IN CULTURE
SYNTHESIS AND SECRETION OF PROTEINS RESEMBLING PLATELET-DERIVED GROWTH-FACTOR BY HUMAN GLIOBLASTOMA AND FIBRO-SARCOMA CELLS IN CULTURE
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DOI：
10.1073/pnas.82.8.2404
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
ANTONIADES, HN
中科院分区：
文献类型：
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作者：
PANTAZIS, P;PELICCI, PG;ANTONIADES, HN
从代谢标记的人胶质母细胞瘤和纤维肉瘤细胞与抗血清血小板衍生生长因子（PDGF）提取的蛋白质的免疫沉淀表明，这些细胞表达和分泌的蛋白质，特异性地识别的抗血清。恶性细胞裂解物中免疫沉淀蛋白质的MW范围为16 [千道尔顿]-140 kDa [千道尔顿]。这两种细胞系分泌的31-kDa的多肽的结构，免疫学和生物学特性相似的人PDGF。这些细胞系合成了一个4.4 kb的mRNA，其中包含了目前鉴定的人类c-sis基因的所有6个外显子的序列。2间充质来源的转化细胞中的PDGF样蛋白至少部分由c-sis基因座编码。
Immunoprecipitation of protein extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The MW of immunoprecipitated proteins in the lysates of the malignant cells ranged from 16 [kilodalton]-140 kDa [kilodalton]. Both cell lines secreted a 31-kDa polypeptide with structural, immunological and biological properties similar to those of human PDGF. These cell lines synthesized a 4.4-kb [kilobase] mRNA that contained sequences from all the 6 currently identified exons of the human c-sis gene. The PDGF-like proteins in the 2 mesenchyme-derived transformed cells are encoded at least in part by the c-sis locus.