Identification and phylogenetic analysis of a glucose transporter gene family from the human pathogenic yeast Candida albicans
Identification and phylogenetic analysis of a glucose transporter gene family from the human pathogenic yeast Candida albicans
复制标题
DOI：
10.1007/s00239-002-2330-4
复制
发表时间：
2002-09-01
影响因子：
3.9
通讯作者：
Shen, SH
中科院分区：
文献类型：
--
作者：
Fan, JJ;Chaturvedi, V;Shen, SH
我们已经确定了一个大家庭的葡萄糖转运蛋白基因（HGT 1至HGT 20）从人类致病性酵母菌白色念珠菌的基因组序列筛选，逆转录PCR检测和系统发育分析。推定的葡萄糖转运蛋白ORF序列在它们之间共享10-93%的成对序列同一性，并且在预测的氨基酸序列的比较分析中，显示出与主要易化剂超家族（MFS）的人和酵母转运蛋白的相似性：所预测的12-跨膜结构域和糖转运蛋白特征与酿酒酵母的HXT转运蛋白和人的GLUT转运蛋白的特征紧密对齐，在某些位置上的氨基酸残基在整个家族中高度保守。逆转录PCR分析表明，大多数的葡萄糖转运蛋白在含有2%葡萄糖的培养基中转录，而几个在低（0.2%）和/或高（5%）浓度的葡萄糖的存在下转录。系统发育分析表明，20个HGT基因可分为3个不同的分支，可能代表3个可能的亚家族。此外，HGT 18和HGT 20显示出与人GLUT的高整体序列同一性，表明可能的功能趋同。我们的结论是，C。白色念珠菌包括一个由20个已知成员组成的家族，该家族具有响应葡萄糖浓度的可变转录。
We have identified a large family of glucose transporter genes (HGT1 to HGT20) from the human pathogenic yeast Candida albicans by screening of genomic sequences, reverse-transcription PCR assays, and phylogenetic analyses. The putative glucose transporter ORF sequences share among themselves 10-93% pairwise sequence identity and, in comparative analyses of predicted amino acid sequences, exhibit similarities to human and yeast transporters of the major facilitator superfamily (MFS): the predicted 12-transmembrane domains and sugar transporter signatures align closely to those of HXT transporters of Saccharomyces cerevisiae and GLUT transporters of humans, with amino acid residues at certain positions highly conserved throughout the families. Reverse-transcription PCR analyses demonstrated that the majority of the glucose transporters was transcribed in culture medium containing 2% glucose, while several were transcribed in the presence of low (0.2%) and/or high (5%) concentrations of glucose. Phylogenetic analyses revealed that there were three distinct clades of 20 HGT genes, which might represent three possible subfamilies. Additionally, HGT18 and HGT20 show a high overall sequence identity to the human GLUTs, indicating a possible functional convergence. We conclude that glucose transporters in C. albicans comprise a family of 20 known members, with variable transcription in response to glucose concentration.