Innervation and receptor profiles of the human apocrine (epitrichial) sweat gland: routes for intervention in bromhidrosis
Innervation and receptor profiles of the human apocrine (epitrichial) sweat gland: routes for intervention in bromhidrosis
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DOI：
10.1111/j.1365-2133.2008.08740.x
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发表时间：
2008-09-01
影响因子：
10.3
通讯作者：
Bovell, D. L.
中科院分区：
文献类型：
--
作者：
Lindsay, S. L.;Holmes, S.;Bovell, D. L.
人类顶泌（毛上）汗腺分泌响应局部或全身给药的儿茶酚胺和胆碱能激动剂。由于人类顶泌腺的分泌过程尚未完全了解，并且没有详细描述肾上腺素能、胆碱能和嘌呤能受体表达的文献，因此需要了解受体类型。这些数据可以提供新的方法来治疗腋臭。目的探讨神经纤维，肾上腺素能，胆碱能和嘌呤能受体在人类腋窝顶泌汗腺免疫组化定位。用免疫组织化学方法检测神经丝、α-和β-肾上腺素受体、P2 Y（1）、P2 Y（2）和P2 Y（4）嘌呤受体以及M-3胆碱受体。顶泌腺的分泌线圈中存在β-2和β-3肾上腺素受体，但不存在α-1、β-1或M-3受体。腺嘌呤能染色（P2 Y（1），P2 Y（2）和P2 Y（4））被发现在看起来像肌上皮细胞，而P2 Y（1）和P2 Y（2）染色被发现在顶端膜和弥漫性整个分泌细胞。外分泌腺染色作为内部阳性对照。结论大汗腺附近没有发现神经纤维，表明任何儿茶酚胺的影响是通过体液作用，腺体可能受到β-肾上腺素受体亚型和嘌呤受体的影响。阻断这两种类型的受体提供了一种途径，以控制顶浆分泌从腋窝腺和减少的机会，为腋臭的发展。
Background Human apocrine (epitrichial) sweat glands secrete in response to local or systemic administration of catecholamines and cholinergic agonists. As the process of secretion in human apocrine glands is not fully understood and no literature detailing the expression of adrenergic, cholinergic and purinergic receptors is available, there is a need to know the receptor types. Such data could provide new approaches for the treatment of axillary bromhidrosis.Objectives To investigate the localization of nerve fibres, adrenergic, cholinergic and purinergic receptors in human axillary apocrine sweat glands by immunohistochemistry.Methods Human axillary apocrine sweat glands were investigated by serial sectioning of paraffin wax-embedded skin samples from volunteers. Sections were examined by light microscopy and immunohistochemistry, using antibodies against neurofilament, alpha- and beta-adrenoceptors, P2Y(1), P2Y(2) and P2Y(4) purinoceptors, and M-3 cholinoceptors.Results Neurofilaments were found near the eccrine but not the apocrine gland. Apocrine glands demonstrated the presence of beta-2 and beta-3 adrenoceptors in the secretory coil of the gland, but not alpha-1, beta-1 or M-3 receptors. Glandular purinergic staining (P2Y(1), P2Y(2) and P2Y(4)) was found in what looked like myoepithelial cells, while P2Y(1) and P2Y(2) staining was found on apical membranes and diffusely throughout secretory cells. Eccrine gland staining acted as internal positive controls.Conclusions No nerve fibres were found near the apocrine gland, suggesting that any catecholamine influence is through humoral effects and that glands could be influenced by beta-adrenoceptor subtypes and purinoceptors. Blockage of both these types of receptors offers a route to controlling apocrine secretion from axillary glands and reducing the opportunity for the development of bromhidrosis.