Genetic variation in structure-function relationships for the inbred mouse lumbar vertebral body
Genetic variation in structure-function relationships for the inbred mouse lumbar vertebral body
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DOI：
10.1359/jbmr.041234
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发表时间：
2005-05-01
影响因子：
6.2
通讯作者：
Jepsen, KJ
中科院分区：
文献类型：
--
作者：
Tommasini, SM;Morgan, TG;Jepsen, KJ
测定了三个近交系小鼠L-5椎体的结构-功能关系。全骨力学特性的遗传变异性可以通过指定皮质和松质骨组织的数量、分布和质量的特征的组合来解释。简介：尽管BMD与骨折的表型相关，但由于BMD不区分潜在的骨形态和成分特征的贡献，因此可能不利于用于遗传和生物力学分析。骨脆性遗传学的研究有助于骨脆性遗传学的研究。材料和方法：用等向体素大小为16µm(3)的A/J、C57BL/6J和C3H/HeJ近交系小鼠L-5椎体(n=10/株)进行微结构和成分测定。使用非接触式应变延伸仪成像系统在压缩状态下测量破坏载荷、刚度和总变形作为延性的量度。骨形态和成分特征与整体骨力学性能的相关性分析。多变量分析确定了每个基因类型的结构-功能关系。结果：没有一个单一的骨骼性状准确地解释了机械性能的遗传差异。然而，描述皮质骨和松质骨组织的数量、分布和质量的特征组合解释了脊椎力学特性变化的70%。结论：不同的遗传背景使用不同的潜在骨特征的不同组合来创造机械功能结构。在遗传分析中，使用单一的复杂性状如BNID或BV/TV作为唯一的表型标记可能被证明是不利的，因为机械性能和潜在的骨特征之间的复杂关系。因此，考虑多种骨骼性状以及这些骨骼性状之间的相互作用对于了解遗传背景与复杂的整体骨力学性质之间的关系是必要的。
Structure-function relationships were determined for L-5 vertebral bodies from three inbred mouse strains. Genetic variability in whole bone mechanical properties could be explained by a combination of the traits specifying the amount, distribution, and quality of the cortical and trabecular bone tissue.Introduction: Although phenotypically correlated with fracture, BMD may be disadvantageous to use in genetic and biomechanical analyses because BMD does not distinguish the contributions of the underlying morphological and compositional bone traits. Developing functional relationships between the underlying bone traits and whole bone mechanical properties should further our understanding of the genetics of bone fragility.Materials and Methods: Microarchitecture and composition of L-5 vertebral bodies (n = 10/strain) from A/J, C57BL/6J, and C3H/HeJ inbred mouse strains were determined using mu CT with an isotropic voxel size of 16 mu m(3). Failure load, stiffness, and total deformation as a measure of ductility were measured in compression using a noncontact strain extensometer imaging system. A correlation analysis related morphological and compositional bone traits to whole bone mechanical properties. A multivariate analysis identified structure-function relationships for each genotype.Results: No single bone trait accurately explained the genetic variation in mechanical properties. However, a combination of traits describing the amount, distribution, and quality of cortical and trabecular bone tissue explained > 70% of the variation in vertebral mechanical properties. Importantly, structure-function relationships were unique among genotypes.Conclusions: Different genetic backgrounds use different combinations of underlying bone traits to create mechanically functional structures. Using a single complex trait such as BNID or BV/TV as the sole phenotypic marker in genetic analyses may prove to be disadvantageous because of the complex relationship between mechanical properties and the underlying bone traits. Therefore, considering multiple bone traits and the interaction among these bone traits is necessary to understand the relationship between genetic background and complex whole bone mechanical properties.