Effects of nonylphenol, bisphenol A, and their mixture on the viviparous swordtail fish (Xiphophorus helleri)
Effects of nonylphenol, bisphenol A, and their mixture on the viviparous swordtail fish (Xiphophorus helleri)
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DOI：
10.1002/etc.5620200414
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发表时间：
2001-04-01
影响因子：
4.1
通讯作者：
Cho, MH
中科院分区：
文献类型：
--
作者：
Kwak, HI;Bae, MO;Cho, MH
一些鱼类物种已被用于内分泌干扰化学品的研究。然而，尽管卵生鱼的广泛使用，相对较少的关注已经给予胎生物种。本研究探讨了内分泌干扰物对一种胎生鱼的影响，并探讨了该鱼作为内分泌干扰物研究的替代模型的可能用途。研究了壬基酚（NP）、双酚A（BPA）及其混合物对剑尾鱼（Xiphophorus helleri）的毒性作用。短期（3天）和相对长期（60天）的曝光进行了成年男性和30日龄的幼鱼，分别。短期暴露后，NP和BPA均引起卵黄蛋白原mRNA的表达。流式细胞仪分析和末端脱氧核苷酸转移酶测定处理后的鱼的睾丸表明生殖损伤。组织学分析发现，暴露于100 ppb NP后，生精小管中的细胞变性和坏死。睾丸病变也与精子发生高度抑制有关。长期暴露后，NP和BPA暴露显着影响剑尾鱼的生长。在所有情况下，结果表明，混合物总是比单一化学品更有效，剑尾鱼可以成为研究内分泌干扰物的有用模型。
A number of fish species have been used for studies on endocrine disrupting chemicals (EDCs). However, despite the widespread use of oviparous fish, relatively little attention has been given to viviparous species. This study investigated the effects of EDCs in a viviparous fish and examined the possible usefulness of the fish as an alternative model for the studies on EDCs. Swordtails (Xiphophorus helleri) were exposed to nonylphenol (NP), bisphenol A (BPA), and their mixture. Both short-term (3-d) and relatively long-term (60-d) exposures were carried out using adult male and 30-d-old juvenile fish, respectively. Following the short-term exposure, both NP and BPA caused vitellogenin mRNA expression. Flow cytometric analysis and terminal deoxynucleotidyl transferase assay on the testes of treated fish indicated reproductive damage. Histopathological analysis found degenerative and necrotic cells in seminiferous tubules following the exposure to 100 ppb NP. The testes with lesions were also associated with highly suppressed spermatogenesis. Following the long-term exposure, both NP and BPA exposures significantly affected the growth of swordtails. In all cases, the results showed that the mixture was always more potent than a single chemical and that swordtail fish can be a useful model for the study of endocrine disruptors.