HNK-1 Glycan Functions as a Tumor Suppressor for Astrocytic Tumor
HNK-1 Glycan Functions as a Tumor Suppressor for Astrocytic Tumor
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DOI：
10.1074/jbc.m111.245886
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发表时间：
2011-09-16
影响因子：
4.8
通讯作者：
Fukuda, Minoru
中科院分区：
文献类型：
--
作者：
Suzuki-Anekoji, Misa;Suzuki, Masami;Fukuda, Minoru
Astrocytic tumor is the most prevalent primary brain tumor. However, the role of cell surface carbohydrates in astrocytic tumor invasion is not known. In a previous study, we showed that polysialic acid facilitates astrocytic tumor invasion and thereby tumor progression. Here, we examined the role of HNK-1 glycan in astrocytic tumor invasion. A Kaplan-Meier analysis of 45 patients revealed that higher HNK-1 expression levels were positively associated with increased survival of patients. To determine the role of HNK-1 glycan, we transfected C6 glioma cells, which lack HNK-1 glycan expression, with beta 1,3-glucuronyltransferase-P cDNA, generating HNK-1-positive cells. When these cells were injected into the mouse brain, the resultant tumors were 60% smaller than tumors emerging from injection of the mock-transfected HNK-1-negative C6 cells. HNK-1-positive C6 cells also grew more slowly than mock-transfected C6 cells in anchorage-dependent and anchorage-independent assays. C6-HNK-1 cells migrated well after treatment of anti-beta 1 integrin antibody, whereas the same treatment inhibited cell migration of mock-transfected C6 cells. Similarly, alpha-dystroglycan containing HNK-1 glycan is different from those containing the laminin-binding glycans, supporting the above conclusion that C6-HNK-1 cells migrate independently from beta 1-integrin-mediated signaling. Moreover, HNK-1-positive cells exhibited attenuated activation of ERK 1/2 compared with mock-transfected C6 cells, whereas focal adhesion kinase activation was equivalent in both cell types. Overall, these results indicate that HNK-1 glycan functions as a tumor suppressor.
