HYBRID GENOMES OF THE POLYOMAVIRUSES JC VIRUS, BK VIRUS, AND SIMIAN VIRUS-40 - IDENTIFICATION OF SEQUENCES IMPORTANT FOR EFFICIENT TRANSFORMATION
HYBRID GENOMES OF THE POLYOMAVIRUSES JC VIRUS, BK VIRUS, AND SIMIAN VIRUS-40 - IDENTIFICATION OF SEQUENCES IMPORTANT FOR EFFICIENT TRANSFORMATION
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DOI：
10.1128/jvi.63.2.863-872.1989
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发表时间：
1989-02-01
影响因子：
5.4
通讯作者：
FRISQUE, RJ
中科院分区：
文献类型：
--
作者：
BOLLAG, B;CHUKE, WF;FRISQUE, RJ
使用杂交病毒基因组来研究特定多瘤病毒序列对 JC 病毒 (JCV) 转化行为的影响。通过交换 JCV 和猿猴病毒 40 (SV40) 或 JCV 和 BK 病毒 (BKV) 之间的调控区，制备了一组嵌合 DNA。产生了第二组构建体，其在 JCV 或 BKV 调节信号的控制下表达杂合 JCV-BKV T 蛋白。用亲本和嵌合DNA转化大鼠2细胞表明，JCV调节信号和编码T蛋白氨基末端的序列均有助于该病毒的受限转化行为。对转化大鼠细胞中病毒蛋白的分析表明，JCV和BKV的大T抗原比其SV40对应物更不稳定，在JCV转化体中产生小t蛋白，并且与细胞p53蛋白形成稳定复合物的T抗原亚群在JCV转化细胞中比在SV40或BKV转化细胞中更小。
Hybrid viral genomes were used to investigate the influence of specific polyomavirus sequences on the transforming behavior of JC virus (JCV). One set of chimeric DNAs was made by exchanging the regulatory regions between JCV and simian virus 40 (SV40) or JCV and BK virus (BKV). A second set of constructs was produced that expressed hybrid JCV-BKV T proteins under the control of either JCV or BKV regulatory signals. Transformation of Rat 2 cells with the parental and chimeric DNAs indicated that both the JCV regulatory signals and the sequence encoding the amino terminus of T protein contributed to the restricted transforming behavior of this virus. Analysis of the viral proteins in the transformed rat cells indicated that the large T antigens of JCV and BKV were less stable than their SV40 counterpart, that small t protein was produced in JCV transformants, and that the subpopulation of T antigen that forms a stable complex with cellular p53 protein was smaller in JCV-transformed cells than in SV40- or BKV-transformed cells.