Demonstration of GlcNAc transferase I in plants.
Demonstration of GlcNAc transferase I in plants.
复制标题
植物中 GlcNAc 转移酶 I 的演示。
DOI：
10.1016/0006-291x(86)90404-3
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发表时间：
1986
影响因子：
3.1
通讯作者：
Elbein,AD
中科院分区：
文献类型：
--
作者：
Szumilo,T;Kaushal,GP;Elbein,AD
来自绿豆幼苗的可溶性酶制剂催化G1cNAc从UDP-G1cNAc转移到Man5G1cNAc受体，形成G1cNAc-Man5G1cNAc。在甘露糖苷酶抑制剂苦马豆素的存在下，低聚糖积累，但在没有该抑制剂的情况下，低聚糖被进一步加工成更小尺寸的低聚糖，同时释放出具有放射性活性的甘露糖。G1cNAc-Man5G1cNAc的形成需要Man5G1cNAc、UDP-G1cNAc、Mn++和苦马豆素的存在。产物G1cNAc-Man5G1cNAc经生物凝胶P-4柱层析和各种酶消化鉴定。这些数据表明植物中存在G1cNAc转移酶I和甘露糖苷酶II。
A solubilized enzyme preparation from mung bean seedlings catalyzed the transfer of G1cNAc from UDP-G1cNAc to the Man5G1cNAc acceptor to form G1cNAc-Man5G1cNAc. In the presence of the mannosidase inhibitor, swainsonine, this oligosaccharide accumulated, but in the absence of this inhibitor, the oligosaccharide was processed further to smaller sized oligosaccharides with the release of radio-active mannose. The formation of G1cNAc-Man5G1cNAc required the presence of Man5G1cNAc, UDP-G1cNAc, Mn++and swainsonine. The product, G1cNAc-Man5G1cNAc was characterized by chromatography on calibrated columns of Biogel P-4, and by various enzymatic digestions. These data indicate the presence of G1cNAc transferase I and mannosidase II in plants.