Does Maternal Body Mass Index Affect the Quantity of Circulating Fetal Cells Available to Use for Cell-Based Noninvasive Prenatal Test in High-Risk Pregnancies?
Does Maternal Body Mass Index Affect the Quantity of Circulating Fetal Cells Available to Use for Cell-Based Noninvasive Prenatal Test in High-Risk Pregnancies?
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DOI：
10.1159/000492028
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发表时间：
2019-01-01
影响因子：
2.2
通讯作者：
Singh, Ripudaman
中科院分区：
文献类型：
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作者：
Kruckow, Sofie;Schelde, Palle;Singh, Ripudaman
我们提出的第一项研究，调查产妇体重指数（BMI）的循环胎儿细胞的数量可用于基于细胞的无创产前检查（cbNIPT）的影响。cbNIPT已被提议作为无细胞胎儿DNA无创产前检测的上级替代方案。Kolvraa et al. [Prenat Diagn. 2016 Dec; 36（12）：1127-34]确定cbNIPT可以在少至一个胎儿细胞上进行，Vestergaard et al. [Prenat Diagn. 2017 Nov; 37（11）：1120-4]证明这些胎儿滋养层细胞可以成功用于cbNIPT以检测染色体和亚染色体异常。这项对91名高危妊娠孕妇的研究表明，cbNIPT不应该受到BMI增加的阻碍，因为每次妊娠，无论BMI如何，都会为下游遗传分析提供胎儿细胞。每个样本的平均胎儿细胞数为12.6，一个样本中的范围为1-43个细胞。方差分析表明，增加母亲的体重指数往往会减少胎儿细胞的数量，但不显着。(C)2018作者（S）由S发布。Karger AG，巴塞尔
We present the first study that investigates the effect of maternal body mass index (BMI) on the quantity of circulating fetal cells available to use in cell-based noninvasive prenatal test (cbNIPT). cbNIPT has been proposed as a superior alternative to noninvasive prenatal test from cell-free fetal DNA. Kolvraa et al. [Prenat Diagn. 2016 Dec; 36(12): 1127-34] established that cbNIPT can be performed on as few as one fetal cell, and Vestergaard et al. [Prenat Diagn. 2017 Nov; 37(11): 1120-4] demonstrated that these fetal trophoblast cells could be used successfully in cbNIPT to detect chromosomal and sub-chromosomal abnormalities. This study on 91 pregnant women with high-risk pregnancies suggests that cbNIPT should not be hampered by an increased BMI because every pregnancy, irrespective of the BMI, has rendered fetal cells for downstream genetic analysis. The mean number of fetal cells per sample was 12.6, with a range of 1-43 cells in one sample. ANOVA showed that increasing maternal BMI tends to decrease the number of fetal cells, but not significantly. (C) 2018 The Author(s) Published by S. Karger AG, Basel