In situ Fourier transform infrared analysis of live cells' response to doxorubicin
In situ Fourier transform infrared analysis of live cells' response to doxorubicin
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DOI：
10.1016/j.bbamcr.2015.07.018
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发表时间：
2015-10-01
影响因子：
5.1
通讯作者：
Chan, K. L. Andrew
中科院分区：
文献类型：
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作者：
Fale, Pedro L.;Altharawi, Ali;Chan, K. L. Andrew
由于某些药物对某些类型的癌症具有特异性，以及某些特定类型的癌症对化疗药物具有耐药性，因此研究癌细胞对化疗药物的反应具有重要意义。我们的目的是开发和应用无标记和非破坏性傅里叶变换红外（FUR）方法来确定三种不同的癌细胞系对不同浓度的常见抗癌药物阿霉素的敏感性，并证明可以从光谱数据中提取有关化疗药物耐药机制的信息。将HeLa、PC3和Caco-2细胞播种在衰减全反射（ATR）晶体上生长，阿霉素在0.1-20 μ M的临床显著浓度下应用，并在20 h内每小时收集一次细胞的光谱。酰胺带分析与细胞活力相关，并与MIT实验交叉验证，从而确定三种细胞系对阿霉素的抗性有显著差异。差异光谱和主成分分析（PCA）强调了处理下活细胞的细微化学变化，分配给核酸（主要是1085 cm(-1)）和碳水化合物（主要是1024 cm(-1)）的光谱区域显示的变化可能与药物的作用方式和细胞系对阿霉素的耐药机制有关。这是一种具有成本效益的方法，不需要生物测定试剂，但允许使用适用于ATR测量的标准FOR设备，对活细胞中的化学变化进行无标签、非破坏性和原位分析。(C) 2015年作者。Elsevier B.V.出版
The study of the response of cancer cells to chemotherapy drugs is of high importance due to the specificity of some drugs to certain types of cancer and the resistance of some specific cancer types to chemotherapy drugs. Our aim was to develop and apply the label-free and non-destructive Fourier transform infrared (FUR) method to determine the sensitivity of three different cancer cell-lines to a common anti-cancer drug doxorubicin at different concentrations and to demonstrate that information about the mechanism of resistance to the chemotherapy drug can be extracted from spectral data. HeLa, PC3, and Caco-2 cells were seeded and grown on an attenuated total reflection (ATR) crystal, doxorubicin was applied at the clinically significant concentration of 0.1-20 mu M, and spectra of the cells were collected hourly over 20 h. Analysis of the amide bands was correlated with cell viability, which had been cross validated with MIT assays, allowing to determine that the three cell lines had significantly different resistance to doxorubicin. The difference spectra and principal component analysis (PCA) highlighted the subtle chemical changes in the living cells under treatment Spectral regions assigned to nucleic acids (mainly 1085 cm(-1)) and carbohydrates (mainly 1024 cm(-1)) showed changes that could be related to the mode of action of the drug and the mechanism of resistance of the cell lines to doxorubicin. This is a costeffective method that does not require bioassay reagents but allows label-free, non-destructive and in situ analysis of chemical changes in live cells, using standard FOR equipment adapted to ATR measurements. (C) 2015 The Authors. Published by Elsevier B.V.