An autoradiographic analysis of [125I]alpha-bungarotoxin binding in rat brain after chronic nicotine exposure.
An autoradiographic analysis of [125I]alpha-bungarotoxin binding in rat brain after chronic nicotine exposure.
复制标题
慢性尼古丁暴露后大鼠脑中[125I]α-银环蛇毒素结合的放射自显影分析。
DOI：
10.1016/j.neulet.2006.05.010
复制
发表时间：
2006
影响因子：
2.5
通讯作者：
Perry,DavidC
中科院分区：
文献类型：
--
作者：
Rasmussen,BruceA;Perry,DavidC
长期接触尼古丁已被证明会增加大鼠大脑中与高亲和力烟碱胆碱能受体的结合，但这种治疗对低亲和力 α7 烟碱受体的影响尚未得到很好的表征。雄性 Sprague-Dawley 大鼠用盐水或尼古丁（6mg/kg/天，通过微型渗透泵）治疗 14 天。然后制备冷冻脑切片并进行处理，使用[125I]α-银环蛇毒素进行定量放射自显影，以测量这种治疗对低亲和力烟碱受体的影响。在分析的 52 个大脑区域中，有 26 个区域的尼古丁暴露增加了 [125I]α-银环蛇毒素结合；与盐水对照相比，增加了 10% 至 70%。大脑的所有区域都出现了增加，但前脑区域更为明显，尤其是大脑皮层。这些数据表明，低亲和力 α7 烟碱受体也会受到慢性尼古丁的上调。这种现象可能与精神分裂症等疾病中大量使用烟草制品有关，并且在针对该受体系统的药物设计中需要考虑到这一现象。
Chronic exposure to nicotine has been shown to increase binding to high affinity nicotinic cholinergic receptors in rat brain, but the effect of this treatment on the low affinity α7 nicotinic receptors has been less well characterized. Male Sprague–Dawley rats were treated with saline or nicotine (6mg/kg/day, by osmotic minipump) for 14 days. Frozen brain sections were then prepared and processed for quantitative autoradiography using [125I]α-bungarotoxin to measure the effect of this treatment on low affinity nicotinic receptors. Nicotine exposure increased [125I]α-bungarotoxin binding in 26 of 52 brain regions analyzed; increases ranged from 10 to 70% over saline controls. Increases were seen in all areas of the brain, but were more prominent in forebrain areas, and especially in cerebral cortex. These data demonstrate that low affinity α7 nicotinic receptors are also up-regulated by chronic nicotine. This phenomenon may be relevant to the heavy use of tobacco products in diseases like schizophrenia, and needs to be considered in the design of pharmaceuticals directed at this receptor system.