The use of citizen science in fish eDNA metabarcoding for evaluating regional biodiversity in a coastal marine region: A pilot study
The use of citizen science in fish eDNA metabarcoding for evaluating regional biodiversity in a coastal marine region: A pilot study
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DOI：
10.3897/mbmg.6.80444
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发表时间：
2022-05-23
影响因子：
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通讯作者：
Fukuchi, Takehiko
中科院分区：
文献类型：
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作者：
Miya, Masaki;Sado, Tetsuya;Fukuchi, Takehiko
为了测试沿海海洋环境中鱼类 eDNA 元条形码公民科学计划的可行性，我们招募了六组自愿公民在自然历史博物馆参加科学教育课程。我们为与会者举办了 eDNA 研讨会以及海水采样和使用注射器和滤芯进行现场过滤的研讨会。之后，他们按照安全实地考察的指南选择了单个调查地点。他们在暑假期间在这些地点进行了海水采样和现场过滤。选定的六个地点出乎意料地包括位于日本中部温带纬度（类似于北纬 35 度）40 公里半径范围内的多样化沿海栖息地。实地考察结束后，他们将过滤后的滤筒返回博物馆，我们从过滤器中提取了 eDNA。我们进行了鱼类 eDNA 元条形码编码以及数据分析。因此，我们从 6 个样本中识别出 66 科 118 属 140 种鱼类，物种丰富度从 14 到 66 种不等。尽管样本量有限，但如此多样化的鱼类分类范围表现出该区域内的空间生物多样性格局，这与物种分布一致。这些包括物种丰富度的南北和城市化梯度、鱼类群落的地理结构以及组成物种不同的盐度偏好。本案例研究展示了鱼类 eDNA 元条形码作为教育和科学工具的潜力，可提高公众意识并开展涵盖区域、国家或全球动物群的大规模公民科学计划。
To test the feasibility of a citizen science program for fish eDNA metabarcoding in coastal marine environments, we recruited six groups of voluntary citizens for a science education course at a natural history museum. We held a seminar on eDNA and a workshop for seawater sampling and on-site filtration using syringes and filter cartridges for the participants. After that, they selected single survey sites following the guidelines for conducting a safe field trip. They performed seawater sampling and on-site filtration at these sites during their summer holidays. The six selected sites unexpectedly included diverse coastal habitats within a 40 km radius, located at temperate latitudes in central Japan (similar to 35 degrees N). After the field trips, they returned filtered cartridges to the museum, and we extracted eDNA from the filters. We performed fish eDNA metabarcoding, along with data analysis. Consequently, we identified 140 fish species across 66 families and 118 genera from the six samples, with species richness ranging from 14 to 66. Despite its limited sample size, such a diverse taxonomic range of fish species exhibited spatial biodiversity patterns within the region, which are consistent with species distribution. These include north-south and urbanization gradients of species richness, geographic structure of the fish communities, and varying salinity preferences of the component species. This case study demonstrates the potential of fish eDNA metabarcoding as an educational and scientific tool to raise public awareness and perform large-scale citizen science initiatives encompassing regional, national, or global fauna.