Central vs. peripheral neuraxial sympathetic control of porcine ventricular electrophysiology.
Central vs. peripheral neuraxial sympathetic control of porcine ventricular electrophysiology.
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猪心室电生理学的中枢与周围神经轴交感神经控制。
DOI：
10.1152/ajpregu.00252.2015
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Mahajan,Aman
中科院分区：
文献类型：
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作者：
Yamakawa,Kentaro;Howard-Quijano,Kimberly;Zhou,Wei;Rajendran,Pradeep;Yagishita,Daigo;Vaseghi,Marmar;Ajijola,OlujimiA;Armour,JAndrew;Shivkumar,Kalyanam;Ardell,JeffreyL;Mahajan,Aman
交感神经兴奋与心室肌纤维化有关。本研究的目的是确定胸背根传入神经输入脊髓在调制正常心脏电生理室交感神经控制的作用。我们假设背根传入输入紧张性地调节心脏的基础和诱发传出交感神经控制。将56个电极的袜子放置在麻醉的约克郡猪（n= 17）的心外膜心室上，记录在基线条件下和星状神经节电刺激期间的电生理功能，以及激活恢复间期（ARI）和ARI的离散度。比较了完整状态和顺序单侧T1-T4背根横断（DRTx）、同侧腹根横断（VRTx）和对侧背根和腹根横断（DVRTx）之间的测量结果。左侧或右侧DRTx降低了基础ARI [左侧DRTx：369 ± 12至319 ± 13 ms（P< 0.01），右侧DRTx：388 ± 19至356 ± 15 ms（P< 0.01）]。随后的单侧VRTx和对侧DRx+VRTx未引起进一步变化。在完整状态下，左和右星状神经节刺激缩短了阿里斯（6 ± 2% vs. 17 ± 3%），同时增加了离散度（+139% vs. +88%）。脊髓根横断后，星状神经刺激的ARI幅度或弥散变化无差异。胸脊髓传入信号的中断导致增强的基底心脏交感神经兴奋性，而不减少交感神经对星状神经节刺激的反应。这表明脊髓背根切断释放脊髓介导的紧张性抑制控制传出交感神经紧张，同时保持胸内心脏为中心的神经网络。
Sympathoexcitation is associated with ventricular arrhythmogenesis. The aim of this study was to determine the role of thoracic dorsal root afferent neural inputs to the spinal cord in modulating ventricular sympathetic control of normal heart electrophysiology. We hypothesize that dorsal root afferent input tonically modulates basal and evoked efferent sympathetic control of the heart. A 56-electrode sock placed on the epicardial ventricle in anesthetized Yorkshire pigs (n= 17) recorded electrophysiological function, as well as activation recovery interval (ARI) and dispersion in ARI, at baseline conditions and during stellate ganglion electrical stimulation. Measures were compared between intact states and sequential unilateral T1–T4 dorsal root transection (DRTx), ipsilateral ventral root transection (VRTx), and contralateral dorsal and ventral root transections (DVRTx). Left or right DRTx decreased global basal ARI [Lt.DRTx: 369 ± 12 to 319 ± 13 ms (P< 0.01) and Rt.DRTx: 388 ± 19 to 356 ± 15 ms (P< 0.01)]. Subsequent unilateral VRTx followed by contralateral DRx+VRTx induced no further change. In intact states, left and right stellate ganglion stimulation shortened ARIs (6 ± 2% vs. 17 ± 3%), while increasing dispersion (+139% vs. +88%). There was no difference in magnitude of ARI or dispersion change with stellate stimulation following spinal root transections. Interruption of thoracic spinal afferent signaling results in enhanced basal cardiac sympathoexcitability without diminishing the sympathetic response to stellate ganglion stimulation. This suggests spinal dorsal root transection releases spinal cord-mediated tonic inhibitory control of efferent sympathetic tone, while maintaining intrathoracic cardiocentric neural networks.