Microfluidics for secretome analysis under enhanced endogenous signaling
Microfluidics for secretome analysis under enhanced endogenous signaling
复制标题
增强内源信号传导下用于分泌蛋白组分析的微流体
DOI：
10.1016/j.bbrc.2018.02.025
复制
发表时间：
2018-03-04
影响因子：
3.1
通讯作者：
Elvassore, Nicola
中科院分区：
文献类型：
--
作者：
Hu, Qianjiang;Luni, Camilla;Elvassore, Nicola
细胞分泌组是由细胞分泌的一组复杂的蛋白质，是体内外细胞间通讯的基本机制。在体内，分泌到体液中的蛋白质的分析可以鉴定重要的生理病理学状况的生物标志物。然而，由于体液的蛋白质含量的复杂性，更好地了解不同细胞类型分泌的蛋白质是非常需要的，并且可以在体外进行解剖。为了这个目的，微流体培养系统可能是特别相关的，因为在微升规模的外源性内源性信号的积累，这更好地保留了在体内的小间隙空间中发生的自我调节。在这项工作中，我们进行了定量研究，以比较微流体和标准孔板中的分泌蛋白。人包皮成纤维细胞被用作案例研究。这项工作也代表了在微流体中高通量定量蛋白质分析的可行性方面的重要技术进步。(C)2018由Elsevier Inc.出版
Cell secretome, the complex set of proteins that are secreted by the cells, is a fundamental mechanism of cell-cell communication both in vitro and in vivo. In vivo, the analysis of proteins secreted into body fluids can bring to the identification of biomarkers for important physiopathological conditions. However, due to the complexity of the protein content of body fluids, a better understanding of the secreted proteins by different cell types is highly desirable and can be performed in vitro for dissection. To this aim, microfluidic culture systems could be particularly relevant because of the accumulation of extrinsic endogenous signals at microliter scale, which better preserves the self-regulation occurring in the small interstitial spaces in vivo. In this work, we perform a quantitative study to compare the secretome in microfluidics and in a standard well plate. Human foreskin fibroblasts are used as a case study. This work also represents an important technological advance in terms of feasibility of high-throughput quantitative protein analyses in microfluidics. (C) 2018 Published by Elsevier Inc.