Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease
Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease
复制标题
DOI：
10.1016/j.nut.2008.12.012
复制
发表时间：
2009-07-01
期刊：
影响因子：
4.4
通讯作者：
Haluzik, Martin
中科院分区：
文献类型：
--
作者：
Roubicek, Tomas;Bartlova, Marketa;Haluzik, Martin
目的：终末期肾病(ESRD)患者的数量正在增加，这些患者患心血管疾病的风险也更高。本研究旨在探讨终末期肾病患者肾移植前脂肪组织激素分泌在慢性炎症发生发展中的作用。方法：15例终末期肾病患者和17例健康女性在手术中(终末期肾病组为肾移植组，对照组为胆囊切除术组)进行单次抽血和内脏及皮下脂肪组织采样。测定血清C-反应蛋白、白介素6、肿瘤坏死因子-α、瘦素、脂联素、抵抗素、单核细胞趋化蛋白-1水平。用实时定量聚合酶链式反应检测皮下和内脏组织中相同激素、脂联素受体1和2以及免疫活性细胞标志物CD68的信使RNA表达。结果：ESRD组血清C-反应蛋白、脂联素、抵抗素、白介素6、肿瘤坏死因子-α和单核细胞趋化蛋白-1水平明显高于对照组。ESRD组大鼠皮下和内脏组织中肿瘤坏死因子-a和CD68的表达均显著高于对照组。ESRD组内脏脂肪组织中脂联素受体-1和单核细胞趋化蛋白-1mRNA的表达显著高于内脏脂肪组织，而皮下脂肪组织中脂联素受体-1和单核细胞趋化蛋白-1mRNA的表达不明显。两组脂肪库中抵抗素、瘦素、脂联素、白介素6和脂联素受体2的信使RNA表达没有显著差异。组织学检查发现，终末期肾病患者皮下和内脏脂肪组织中CD68阳性免疫活性细胞增多。结论：终末期肾病患者皮下和内脏脂肪组织表达较高水平的促炎细胞因子，可能在全身炎症的发生发展中起一定作用。(C)2009 Elsevier Inc.保留所有权利。
Objective: The number of patients with end-stage renal disease (ESRD) is rising and these patients are at higher risk of cardiovascular disease. We studied the role of hormonal production of adipose tissue in the development of chronic inflammation in patients with ESRD before kidney transplantation.Methods: Fifteen women with ESRD and 17 healthy women (control) underwent single blood drawing and visceral and subcutaneous adipose tissue sampling during surgery (kidney transplantation in the ESRD group or cholecystectomy in the control group). Serum concentrations of C-reactive protein, interleukin-6, tumor necrosis factor-alpha, leptin, adiponectin, resistin, monocyte chemoattractant protein-1 were measured. Messenger RNA expression of the same hormones, adiponectin receptors 1 and 2 and immunocompetent cell marker CD68 in subcutaneous and visceral samples were measured using real-time polymerase chain reaction. Adipose tissue was examined immunohistochemically for CD68-positive cells.Results: Serum concentrations of C-reactive protein, adiponectin, resistin, interleukin-6, tumor necrosis factor-a, and monocyte chemoattractant protein-1 were significantly higher in the ESRD versus control group. Subcutaneous and visceral mRNA expressions of tumor necrosis factor-a and CD68 were significantly increased in the ESRD versus control group. Adiponectin receptor-1 and monocyte chemoattractant protein-1 mRNA expressions were significantly higher in visceral but not in subcutaneous adipose tissue of the ESRD group. Messenger RNA expressions of resistin, leptin, adiponectin, interleukin-6, and adiponectin receptor-2 in both fat depots did not significantly differ between groups. Increased infiltration of subcutaneous and visceral adipose tissue with CD68-positive immunocompetent cells was found in the ESRD group by histologic examination.Conclusion: Subcutaneous and visceral adipose tissues in ESRD express higher amounts of proinflammatory cytokines and may play a role in the development of systemic inflammation. (C) 2009 Elsevier Inc. All rights reserved.