Differential coherent anti-Stokes Raman scattering microscopy with linearly chirped femtosecond laser pulses.
Differential coherent anti-Stokes Raman scattering microscopy with linearly chirped femtosecond laser pulses.
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DOI：
10.1364/ol.34.002258
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发表时间：
2009-08
期刊：
影响因子：
3.6
通讯作者：
I. Rocha-Mendoza;W. Langbein;P. Watson;P. Borri
中科院分区：
文献类型：
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作者：
I. Rocha-Mendoza;W. Langbein;P. Watson;P. Borri
我们展示了一种利用线性啁啾飞秒激光脉冲进行差分相干反斯托克斯拉曼散射(CARS)显微镜的技术。通过复制激发的泵浦-斯托克斯脉冲对以两倍于激光重复频率的速度产生脉冲序列，并通过玻璃色散控制每对脉冲的瞬时频差，我们可以以一种本质上稳定且经济高效的方式来调整每对脉冲探测到的拉曼频率。由此产生的CARS强度由单个光电倍增管使用相敏频率滤波检测为和和差。我们展示了聚合物微珠和活细胞的成像，抑制了非共振CARS背景，提高了化学敏感性。
We demonstrate a technique for differential coherent anti-Stokes Raman scattering (CARS) microscopy employing linearly chirped femtosecond laser pulses. By replicating the exciting pump-Stokes pulse pairs to create a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference of each pair by glass dispersion, we can adjust the Raman frequency probed by each pair in an intrinsically stable and cost-effective way. The resulting CARS intensities are detected by a single photomultiplier as sum and difference using phase-sensitive frequency filtering. We demonstrate imaging of polymer beads and living cells with suppressed nonresonant CARS background and improved chemical sensitivity.