A quantitative assay for lycopene that utilizes reduced volumes of organic solvents
A quantitative assay for lycopene that utilizes reduced volumes of organic solvents
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DOI：
10.1006/jfca.2002.1069
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发表时间：
2002-06-01
影响因子：
4.3
通讯作者：
Collins, JK
中科院分区：
文献类型：
--
作者：
Fish, WW;Perkins-Veazie, P;Collins, JK
番茄红素是一种有效的抗氧化剂，存在于西瓜、番茄和红葡萄柚中，可能对人体健康有积极影响。分光光度法和高效液相色谱法是食品中番茄红素含量分析的常用方法。提出了一种快速、廉价的番茄红素分光光度测定方法。与现有方法相比，该方法从西瓜中释放和提取番茄红素所需的有机溶剂减少了80%。对来自11个品种的105个西瓜进行了比较分析，结果与大体积分光光度法的结果相当。有限的试验表明，这种减少体积的方法可能适用于番茄和番茄制品。Elsevier Science Ltd.出版。
Lycopene is a potent antioxidant found in watermelon, tomato, and red grapefruit and may exert positive effects on human health. Spectrophotometric and HPLC techniques are commonly employed for analysis of lycopene content in food sources. A rapid and inexpensive spectrophotometric assay for lycopene is presented. This method requires 80% less organic solvents for release and extraction of lycopene from watermelon than do the existing procedures. Comparative analyses for 105 watermelons from 11 cultivars yielded results equivalent to those provided by larger-volume spectrophotometric assay procedures, Limited numbers of assays suggest that this reduced volume method may be applicable for tomatoes and tomato products. Published by Elsevier Science Ltd.