A novel microbial habitat of alkaline black liquor with very high pollution load: microbial diversity and the key members in application potentials.
A novel microbial habitat of alkaline black liquor with very high pollution load: microbial diversity and the key members in application potentials.
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DOI：
10.1016/j.biortech.2009.09.092
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发表时间：
2010-03
影响因子：
11.4
通讯作者：
Chunyu Yang;Y. Niu;Haijun Su;Zhe Wang;Fei Tao;Xia Wang;Hongzhi Tang;Cuiqing Ma;P. Xu
中科院分区：
文献类型：
--
作者：
Chunyu Yang;Y. Niu;Haijun Su;Zhe Wang;Fei Tao;Xia Wang;Hongzhi Tang;Cuiqing Ma;P. Xu
采用培养和非培养技术对碱性造纸黑液中自然生长的微生物群落进行了研究。该群落对黑液pH值、色度和化学需氧量（COD）均有较好的降低作用，其社群活动随季节变化而变化。16 S rDNA克隆文库和PCR-DGGE分析表明，不同季节的细菌群落在种类和组成上存在明显差异。梭状芽孢杆菌是造纸黑液处理的关键菌种。此外，盐单胞菌属和芽孢杆菌属的菌株被证明是有效的，在处理非常严重的污染黑液。盐单胞菌属、梭菌属和芽孢杆菌属可能是该群落中木聚糖酶和羧甲基纤维素酶的主要生产菌。这些微生物的应用潜力将使造纸黑液处理和再生资源利用成为世界性难题。
A microbial community which developed naturally in alkaline black liquor was investigated by culture-based and culture-independent techniques. The community was effective in lowering pH, color, and chemical oxygen demand (COD) of black liquor, and the community activities varied in different seasons. Both 16S ribosomal DNA (rDNA) clone library and polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE) analyses suggested that the seasonal bacterial communities had obvious differences in diversities and compositions. Clostridium species were suggested to be the key agents in black liquor treatment. Moreover, the isolates of the genera Halomonas and Bacillus were shown to be effective in treating very heavily polluted black liquor. The strains of Halomonas, Clostridium and especially Bacillus, might be the key producers of xylanase and CMCase in the community. The worldwide problem of black liquor treatment and renewable resource utilization would benefit from these microorganisms in application potentials.