Structure of transcribing RNA polymerase II-nucleosome complex
Structure of transcribing RNA polymerase II-nucleosome complex
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DOI：
10.1038/s41467-018-07870-y
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发表时间：
2018-12-21
影响因子：
16.6
通讯作者：
Cramer, Patrick
中科院分区：
文献类型：
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作者：
Farnung, Lucas;Vos, Seychelle M.;Cramer, Patrick
真核蛋白编码基因的转录需要RNA聚合酶II（Pol II）通过核小体，但目前尚不清楚这是如何实现的。在这里，我们报告的cryo-EM结构的转录酿酒酵母Pol II从事与下游核小体核心颗粒在4.4埃的整体分辨率。Pol II和核小体以未预测的确定的相对方向观察到。Pol II使用其钳头和叶结构域接触核小体二分体的两侧。结构比较表明，延伸因子TFIIS，DSIF，NELF，SPT 6和PAF 1复合物可以容纳在Pol II表面上的定向核小体的存在下。我们的研究结果为分析染色质转录的机制提供了一个起点。
Transcription of eukaryotic protein-coding genes requires passage of RNA polymerase II (Pol II) through nucleosomes, but it is unclear how this is achieved. Here we report the cryo-EM structure of transcribing Saccharomyces cerevisiae Pol II engaged with a downstream nucleosome core particle at an overall resolution of 4.4 angstrom. Pol II and the nucleosome are observed in a defined relative orientation that is not predicted. Pol II contacts both sides of the nucleosome dyad using its clamp head and lobe domains. Structural comparisons reveal that the elongation factors TFIIS, DSIF, NELF, SPT6, and PAF1 complex can be accommodated on the Pol II surface in the presence of the oriented nucleosome. Our results provide a starting point for analysing the mechanisms of chromatin transcription.