Expression profiling of the lignin biosynthetic pathway in Norway spruce using EST sequencing and real-time RT-PCR
Expression profiling of the lignin biosynthetic pathway in Norway spruce using EST sequencing and real-time RT-PCR
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DOI：
10.1007/s11103-007-9220-5
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发表时间：
2007-10-01
影响因子：
5.1
通讯作者：
Teeri, Teemu H.
中科院分区：
文献类型：
--
作者：
Koutaniemi, Sanna;Warinowski, Tino;Teeri, Teemu H.
木质素的生物合成是裸子植物和木本被子植物的主要碳汇。许多涉及的酶是由几个基因编码的，其中一些基因也与其他苯丙素的生物合成有关。在本研究中，我们旨在鉴定挪威云杉（Picea abies (L.)）中负责发育木质素化的基因家族成员。岩溶)。利用EST测序和实时定量RT-PCR分析了木质素整个生物合成途径的基因表达。还研究了弯曲应力和杂交菌侵染下的应力诱导木质素化。从云杉木质素形成组织培养和木质部发育过程中，共测序了7189条ESTs序列，并将其聚类到3831个单基因中。在单脂醇生物合成酶和聚合相关酶中发现了几个同源基因。实时RT-PCR结果突出了一组单分子生物合成基因，可能负责挪威云杉的发育木质素化。还鉴定了单脂醇聚合的潜在基因。压缩木材中表达的单木质素生物合成基因集基本相同，但过氧化物酶的表达与垂直生长的对照不同。病原菌感染韧皮部导致单脂醇生物合成途径的普遍上调，并诱导了一些新的基因家族成员。从病原菌侵害人和压缩木材中PaPAL2、PaPX2和PaPX3的表达上调来看，PaPAL2、PaPX2和PaPX3具有普遍的胁迫诱导功能。
Lignin biosynthesis is a major carbon sink in gymnosperms and woody angiosperms. Many of the enzymes involved are encoded for by several genes, some of which are also related to the biosynthesis of other phenylpropanoids. In this study, we aimed at the identification of those gene family members that are responsible for developmental lignification in Norway spruce (Picea abies (L.) Karst.). Gene expression across the whole lignin biosynthetic pathway was profiled using EST sequencing and quantitative real-time RT-PCR. Stress-induced lignification during bending stress and Heterobasidion annosum infection was also studied. Altogether 7,189 ESTs were sequenced from a lignin forming tissue culture and developing xylem of spruce, and clustered into 3,831 unigenes. Several paralogous genes were found for both monolignol biosynthetic and polymerisation-related enzymes. Real-time RT-PCR results highlighted the set of monolignol biosynthetic genes that are likely to be responsible for developmental lignification in Norway spruce. Potential genes for monolignol polymerisation were also identified. In compression wood, mostly the same monolignol biosynthetic gene set was expressed, but peroxidase expression differed from the vertically grown control. Pathogen infection in phloem resulted in a general up-regulation of the monolignol biosynthetic pathway, and in an induction of a few new gene family members. Based on the up-regulation under both pathogen attack and in compression wood, PaPAL2, PaPX2 and PaPX3 appeared to have a general stress-induced function.