Comparative genomics and gene-trait matching analysis of Bifidobacterium breve from Chinese children
Comparative genomics and gene-trait matching analysis of Bifidobacterium breve from Chinese children
复制标题
中国儿童短双歧杆菌比较基因组学及基因性状匹配分析
DOI：
10.1016/j.fbio.2020.100631
复制
发表时间：
2020-08-01
期刊：
影响因子：
5.2
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Liu, Rui;Yang, Bo;Chen, Wei
短双歧杆菌是儿童双歧杆菌的主要种类之一。本研究利用全基因组测序和比较基因组学技术，对从中国儿童粪便中分离的46株短芽孢杆菌进行了遗传多样性分析，并对其碳水化合物利用和抗生素耐受性进行了基因型和表型分析。系统发育树不受地域、年龄和饲养方式的影响，在遗传水平上碳水化合物和抗生素的聚类没有规律性。基于基因型-表型相关分析，糖基水解酶的多样性和菌株代谢碳水化合物的能力证实了短芽胞杆菌在儿童肠道中的优势。同时，菌株对抗生素的敏感性增加了对其遗传特征的认识，为安全性评价提供了潜在的依据。
Bifidobacterium breve is one of the dominant Bifidobacterial species in children. In the current work, 46 strains of B. breve isolated from fecal samples of Chinese children were analyzed using whole-genome sequencing and comparative genomics to explore their genetic diversity, as well as genotype and phenotype analysis for carbohydrate utilization and antibiotic tolerance. The phylogenetic tree was independent of region, age and feeding mode, and without any regularity in the clustering of carbohydrates and antibiotics at the genetic level. Based on genotypic-phenotypic correlation analysis, the diversity of glycosyl hydrolases and the ability of strains to metabolize carbohydrates corroborated the predominance of B. breve in the children's intestines. Simultaneously, the sensitivity of strains to antibiotics increased the understanding of its genetic features and provided a potential basis for safety evaluation.