Positioning scission of single DNA molecules with nonspecific endonuclease based on nanomanipulation
Positioning scission of single DNA molecules with nonspecific endonuclease based on nanomanipulation
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基于纳米操作的非特异性核酸内切酶定位单个 DNA 分子的断裂
DOI：
10.1021/ja0687015
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发表时间：
2007-05-30
影响因子：
15
通讯作者：
Hu, Jun
中科院分区：
文献类型：
--
作者：
Li, Bin;Zhang, Yi;Hu, Jun
在单分子水平上控制纳米级化学和生化反应引起了人们的极大兴趣，因为它可以探索传统本体系统无法实现的新现象和/或揭示新机制。在本文中，我们报告了 DNase I（一种众所周知且广泛使用的非特异性核酸内切酶）如何通过使用称为组合动态浸笔纳米光刻 (CDDPN) 的方法在受控位点消化 DNA。即使在表面环境条件下也能实现高效消化。我们将这种现象归因于 DNase I 被限制在纳米点中时的高有效浓度。
Controlling chemical and biochemical reactions on nanoscale at the single-molecule level is of great interest due to its possibility for exploring new phenomena and/or unraveling novel mechanisms that are inaccessible for traditional bulk systems. In this paper, we report how DNase I, a well-known and widely used nonspecific endonuclease, digested DNA at controlled sites by using an approach termed combined-dynamic dip-pen nanolithography (CDDPN). High efficient digestion has been realized even under an ambient condition on the surfaces. We attribute this phenomenon to a high effective concentration of DNase I when confined in the nanospots.