Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila
Squid, Cup, and PABP55B function together to regulate gurken translation in Drosophila
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DOI：
10.1016/j.ydbio.2007.11.008
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发表时间：
2008-01-15
影响因子：
2.7
通讯作者：
Schupbach, Trudi
中科院分区：
文献类型：
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作者：
Clouse, K. Nicole;Ferguson, Scott B.;Schupbach, Trudi
在果蝇卵子发生过程中，gurken（grk）mRNA和蛋白质的正确定位对于卵和胚胎背腹轴的形成是必需的。Squid（Sqd）是一种RNA结合蛋白，其是grk信息的正确定位和翻译调节所必需的。我们表明，杯和polyA结合蛋白（PABP）的物理相互作用与Sqd和彼此在卵巢。我们发现，杯突变体奠定dorsalized鸡蛋，增强dorsalization弱sqd等位基因，并显示缺陷的grk mRNA定位和Grk蛋白积累。相反，pAbp突变体产腹侧卵并增强grk单倍不足。PABP还与安可发生遗传和生物化学相互作用。这些数据预测了一个模型，其中杯和Sqd介导的翻译抑制未定位的grk mRNA，和PABP和埃里克促进翻译激活的消息，一旦它是完全本地化的背前部区域的卵母细胞。这些数据还提供了第一个证据，证明了常用的反式作用因子复合物与编码蛋白（一种grk翻译所需的因子）之间存在联系。(C)2007年爱思唯尔公司All rights reserved.
During Drosophila melanogaster oogenesis, the proper localization of gurken (grk) mRNA and protein is required for the establishment of the dorsal-ventral axis of the egg and future embryo. Squid (Sqd) is an RNA-binding protein that is required for the correct localization and translational regulation of the grk message. We show that Cup and polyA-binding protein (PABP) interact physically with Sqd and with each other in ovaries. We show that cup mutants lay dorsalized eggs, enhance dorsalization of weak sqd alleles, and display defects in grk mRNA localization and Grk protein accumulation. In contrast, pAbp mutants lay ventralized eggs and enhance grk haploinsufficiency. PABP also interacts genetically and biochemically with Encore. These data predict a model in which Cup and Sqd mediate translational repression of unlocalized grk mRNA, and PABP and Eric facilitate translational activation of the message once it is fully localized to the dorsal-anterior region of the oocyte. These data also provide the first evidence of a link between the complex of commonly used trans-acting factors and Enc, a factor that is required for grk translation. (C) 2007 Elsevier Inc. All rights reserved.