Validation of Clausius-Mossotti Function in Wideband Single-Cell Dielectrophoresis
Validation of Clausius-Mossotti Function in Wideband Single-Cell Dielectrophoresis
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DOI：
10.1109/jerm.2019.2894100
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发表时间：
2019-06-01
影响因子：
3.2
通讯作者：
Hwang, James C. M.
中科院分区：
文献类型：
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作者：
Du, Xiaotian;Ma, Xiao;Hwang, James C. M.
对于第一次，无论是较低的和较高的交叉频率的真实的部分的Clausius-Mossotti函数计算通过使用细胞参数测量在相同的超宽带设置为单细胞介电电泳。计算表明，在不太可能的情况下，当电池参数的不确定性全部加起来时，下交叉频率可以从0到127 kHz，上交叉频率可以从45到108 MHz。计算出的较低交叉频率与28 +/- 4 kHz的测量值基本一致。然而，计算的上交越频率与326 +/- 35 MHz的测量值存在显著差异。这种差异可以归因于场在单细胞介电泳中是高度不均匀的，特别是在较高频率下。此外，在单细胞介电电泳中使用紧密间隔的电极，可能必须考虑粘附力，即使对于相对非粘附的Jurkat细胞。在任何情况下，计算和测量的交叉频率之间的差异表明，经典的Clausius-Mossotti函数，最初来自麦克斯韦-瓦格纳混合模型的细胞悬浮液，可能不适用于单细胞介电电泳在一个简单的方式，特别是在高频率。
For the first time, both lower and upper crossover frequencies of the real part of the Clausius-Mossotti function were calculated by using cell parameters measured on the same ultrawideband setup as that for single-cell dielectrophoresis. The calculation suggests that the lower crossover frequency can be from 0 to 127 kHz and that the upper crossover frequency can be from 45 to 108 MHz, in the unlikely case when uncertainties in the cell parameters all add up. The calculated lower crossover frequency was found to be in general agreement with the measured values of 28 +/- 4 kHz. However, the calculated upper crossover frequency was significantly different from the measured values of 326 +/- 35 MHz. The difference can be attributed to the field being highly nonuniform in single-cell dielectrophoresis, especially at higher frequencies. Additionally, with closely spaced electrodes in single-cell dielectrophoresis, adhesive force may have to be considered, even for a relatively nonadherent Jurkat cell. In any case, the difference between the calculated and measured crossover frequency suggests that the classical Clausius-Mossotti function, originally derived from the Maxwell-Wagner mixture model of a cell suspension, may not apply to single-cell dielectrophoresis in a straightforward manner, especially at high frequencies.