Simultaneous live imaging of the transcription and nuclear position of specific genes.
Simultaneous live imaging of the transcription and nuclear position of specific genes.
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特定基因的转录和核位置的同时实时成像。
DOI：
10.1093/nar/gkv624
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发表时间：
2015-10-30
影响因子：
14.9
通讯作者：
Yamamoto T
中科院分区：
文献类型：
--
作者：
Ochiai H;Sugawara T;Yamamoto T
最近已经建立了基因组组织和基因表达之间的关系。然而，基因组的空间组织、动力学和转录调控之间的关系仍然未知。在这项研究中，我们开发了一种实时成像方法，用于同时测量内源基因的转录活性和核位置，我们将其称为“定位和表达实时观察（ROLEX）”系统。我们证明ROLEX具有高度特异性，不会影响目标基因的表达水平。 ROLEX 能够检测取决于胚胎干细胞中 Nanog 反式激活状态的亚基因组范围的迁移性变化。我们相信ROLEX系统将成为探索活细胞转录与核动力学关系的有力工具。
The relationship between genome organization and gene expression has recently been established. However, the relationships between spatial organization, dynamics, and transcriptional regulation of the genome remain unknown. In this study, we developed a live-imaging method for simultaneous measurements of the transcriptional activity and nuclear position of endogenous genes, which we termed the ‘Real-time Observation of Localization and EXpression (ROLEX)’ system. We demonstrated that ROLEX is highly specific and does not affect the expression level of the target gene. ROLEX enabled detection of sub-genome-wide mobility changes that depended on the state of Nanog transactivation in embryonic stem cells. We believe that the ROLEX system will become a powerful tool for exploring the relationship between transcription and nuclear dynamics in living cells.