MicroRNA-217, down-regulated in clear cell renal cell carcinoma and associated with lower survival, suppresses cell proliferation and migration
MicroRNA-217, down-regulated in clear cell renal cell carcinoma and associated with lower survival, suppresses cell proliferation and migration
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DOI：
10.4149/neo_2013_066
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发表时间：
2013-01-01
期刊：
影响因子：
3
通讯作者：
Ma, W. M.
中科院分区：
文献类型：
--
作者：
Li, H.;Zhao, J.;Ma, W. M.
异常表达的microRNAs(MiRNAs)经常与多种人类癌症相关，包括肾透明细胞癌(CcRCC)。在本研究中，我们确定了miR-217在肾细胞癌中的表达模式，并检测了其对癌细胞增殖和迁移的影响。采用实时定量定量聚合酶链式反应技术检测54例肾细胞癌组织中miR-217的表达水平。将miR-217模拟物和miRNA模拟物分别导入786-O和ACHN细胞。用四甲基偶氮唑盐比色法和划痕试验检测细胞的增殖和迁移。我们发现，与配对的正常组织相比，miR-217在肾细胞癌中下调。MiR-217的低表达水平与肿瘤的分级和分期有关。MiR-217高表达的患者全部存活5年，而miR-217低表达的患者仅40%存活。转染miR-217模拟物的细胞增殖受到抑制，运动能力下降。综上所述，miR-217在肾细胞癌中具有肿瘤抑制作用。
Aberrantly expressed microRNAs (miRNAs) are frequently correlated with a variety of human cancers, including clear cell renal cell carcinoma (ccRCC). In this study, we determined the expression patterns of miR-217 in ccRCC, and tested its effect on cancer cell proliferation and migration. The expression levels of miR-217 were determined in 54 ccRCC samples using Real-Time qPCR. 786-O and ACHN cells were transfected with miR-217 mimics or miRNA mimics control. Cell proliferation and migration were evaluated by MTT assay and scratch-wound assay, respectively. We found that miR-217 was down-regulated in ccRCC compared to paired normal tissue. Lower miR-217 expression levels were associated with higher tumor grade and stage. All patients with high miR-217 expression survived 5 years, while with low miR-217 expression, only 40% survived. Cell proliferation inhibition and decreased motility were observed in cells transfected with the miR-217 mimics. In conclusion, miR-217 plays a tumor suppressor role in ccRCC.