CARD-FISH analysis of prokaryotic community composition and abundance along small-scale vegetation gradients in a dry arctic tundra ecosystem
CARD-FISH analysis of prokaryotic community composition and abundance along small-scale vegetation gradients in a dry arctic tundra ecosystem
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DOI：
10.1016/j.soilbio.2013.05.002
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发表时间：
2013-09-01
影响因子：
9.7
通讯作者：
Nakano, Shin-ichi
中科院分区：
文献类型：
--
作者：
Ushio, Masayuki;Makoto, Kobayashi;Nakano, Shin-ichi
土壤微生物群落的大小和组成对土壤分解等陆地生态系统过程有重要影响。然而，与地上植物群落的研究相比，对北极地区地下微生物群落及其与地上植被相互作用的研究相对较少。在这项研究中，我们进行了第一次调查的丰度和组成的原核生物群落沿着小尺度植被梯度（约。1-3 m）在瑞典北方的一个干旱的北极苔原生态系统中，使用荧光原位杂交（FISH）结合催化报告沉积（CARD）。原核细胞的数量增加，植被覆盖沿着植被梯度，主要是作为一个函数的增加量的土壤碳和水分。真细菌和假单胞菌分别占DAPI阳性细胞的59.7%和33.4%。在所分析的细菌门和亚门中，酸杆菌和α-变形菌是最主要的类群，分别占DAPI阳性细胞的约13.5%和10.7%。有趣的是，土壤原核生物群落组成相对不受地上植被群落剧烈变化的影响。多变量分析表明，原核生物群落组成取决于土壤pH值，而不是地上植被。在所研究的土壤系统中，地表植物是土壤微生物群落组成的弱预测因子，群落的大小受碳和水的可用性的限制。此外，我们的研究表明，CARD-FISH，这仍然是一个很少使用的技术在土壤生态学，是有效的量化土壤微生物。(C)2013爱思唯尔有限公司保留所有权利。
The size and composition of soil microbial communities have important influences on terrestrial ecosystem processes such as soil decomposition. However, compared with studies of aboveground plant communities, there are relatively few studies on belowground microbial communities and their interactions with aboveground vegetations in the arctic region. In this study, we conducted the first investigation of the abundance and composition of prokaryotic communities along small-scale vegetation gradients (ca. 1-3 m) in a dry arctic tundra ecosystem in Northern Sweden using fluorescent in situ hybridization (FISH) coupled with catalyzed reporter deposition (CARD). The number of prokaryotic cells increased with increasing vegetation cover along vegetation gradients, mainly as a function of increased amounts of soil carbon and moisture. Eubacteria and Archaea constituted approximately 59.7% and 33.4% of DAPI-positive cells, respectively. Among the analyzed bacterial phyla and sub-phyla, Acidobacteria and alpha-proteobacteria were the most dominant groups, constituting approximately 13.5% and 10.7% of DAPI-positive cells, respectively. Interestingly, the soil prokaryotic community composition was relatively unaffected by the dramatic changes in the aboveground vegetation community. Multivariate analyses suggested that the prokaryotic community composition depended on soil pH rather than on aboveground vegetation. Surface plants are weak predictors of the composition of the soil microbial community in the studied soil system and the size of the community is constrained by carbon and water availability. In addition, our study demonstrated that CARD-FISH, which is still a rarely-used technique in soil ecology, is effective for quantifying soil microbes. (C) 2013 Elsevier Ltd. All rights reserved.