Surface-enhanced Raman nanoparticle beacons based on bioconjugated gold nanocrystals and long range plasmonic coupling.
Surface-enhanced Raman nanoparticle beacons based on bioconjugated gold nanocrystals and long range plasmonic coupling.
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DOI：
10.1021/ja8062502
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发表时间：
2008-11-12
影响因子：
15
通讯作者：
Nie, Shuming
中科院分区：
文献类型：
--
作者：
Qian, Ximei;Zhou, Xi;Nie, Shuming
我们开发了一种新型的表面增强拉曼散射信标(SERS信标)，它可以通过生物分子识别和结合事件引发的远程等离子体耦合来开启和关闭。信标是基于两种尺寸(40 nm和60 nm)的胶体金纳米晶体，通过拉曼报告分子的光谱编码制备的，用硫代化的DNA探针功能化，并由低分子量聚乙二醇组分稳定和保护。结果表明，当纳米粒子信标被等离子体耦合激活时，SERS信号强度增加了40-200倍，远高于传统分子信标报道的明暗比。多价金纳米颗粒也具有精致的特异性，能够识别单碱基错配或突变。这类SERS纳米粒子信标具有新颖的分子检测和信号放大机制，其长程耦合特性为开发等离子体探针检测蛋白质、细胞和完整病毒提供了新的机会。
We have developed a new class of surface-enhanced Raman scattering beacons (SERS beacons) that can be turned on and off by long-range plasmonic coupling, induced by biomolecular recognition and binding events. The beacons are based on colloidal gold nanocrystals in two sizes (40 nm and 60 nm), and are prepared by spectral encoding with a Raman reporter molecule, functionalized with thiolated DNA probes, and stabilized and protected by low-molecular weight polyethylene glycols (PEG). The results show the SERS signal intensities increase by 40-200 fold when the nanoparticle beacons are activated by plasmonic coupling, much higher than the bright-to-dark intensity ratios reported for traditional molecular beacons. Multivalent gold nanoparticles also have exquisite specificity and are able to recognize single-base mismatches or mutations. This class of SERS nanoparticle beacons has novel mechanisms for molecular detection and signal amplification, and its long-range coupling nature raises new opportunities in developing plasmonic probes to detect proteins, cells, and intact viruses.
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