Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells.
Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells.
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DOI：
10.4049/jimmunol.151.10.5328
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发表时间：
1993-11
影响因子：
4.4
通讯作者：
P. A. Mathew;B. Garni-Wagner;K. Land;A. Takashima;E. Stoneman;M. Bennett;V. Kumar
中科院分区：
文献类型：
--
作者：
P. A. Mathew;B. Garni-Wagner;K. Land;A. Takashima;E. Stoneman;M. Bennett;V. Kumar
我们最近描述了一种信号转导分子 2B4，它在所有介导非 MHC 限制性杀伤的 NK 和 T 细胞上表达。克隆了编码该分子的基因并确定了其核苷酸序列。编码的蛋白质由398个氨基酸组成，前导肽由18个氨基酸组成，跨膜区由24个氨基酸组成。预测的蛋白质有八个 N 连接糖基化位点，表明它是高度糖基化的。将2B4与数据库中的序列进行比较表明2B4是Ig超基因家族的成员，并且与小鼠和大鼠CD48以及人LFA-3显示出同源性。 Northern 印迹分析显示，在几种小鼠品系的贴壁淋巴因子激活杀伤细胞和 TCR-γ/δ 树突状表皮 T 细胞系中至少有 3 个 2B4 转录本，但在同种异体特异性 T 细胞克隆中没有。这三种mRNA是异源核RNA差异剪接的产物。对几种小鼠品系的基因组 DNA 进行 Southern 印迹分析表明，2B4 属于密切相关的基因家族。通过分析重组近交小鼠品系中的 2B4 表达，已将 2B4 基因定位到小鼠 1 号染色体。
We have recently described a signal transducing molecule, 2B4, expressed on all NK and T cells that mediate non-MHC-restricted killing. The gene encoding this molecule was cloned and its nucleotide sequence determined. The encoded protein of 398 amino acids has a leader peptide of 18 amino acids and a transmembrane region of 24 amino acids. The predicted protein has eight N-linked glycosylation sites, suggesting that it is highly glycosylated. Comparison of 2B4 with sequences in the databanks indicates that 2B4 is a member of Ig supergene family, and it shows homology to murine and rat CD48 and human LFA-3. Northern blot analysis has shown at least three transcripts for 2B4 in adherent lymphokine-activated killer cells of several mouse strains and TCR-gamma/delta dendritic epidermal T cell lines but not in allospecific T cell clones. These three mRNA are the products of differential splicing of heterogeneous nuclear RNA. Southern blot analysis of genomic DNA from several mouse strains revealed that 2B4 belongs to a family of closely related genes. The 2B4 gene has been mapped to mouse chromosome 1 by analysis of 2B4 expression in recombinant inbred mouse strains.