SYSTEMS UTILIZING OXYGENATED LEGHEMOGLOBIN AND MYOGLOBIN AS SOURCES OF FREE DISSOLVED O2 AT LOW CONCENTRATIONS FOR EXPERIMENTS WITH BACTERIA
SYSTEMS UTILIZING OXYGENATED LEGHEMOGLOBIN AND MYOGLOBIN AS SOURCES OF FREE DISSOLVED O2 AT LOW CONCENTRATIONS FOR EXPERIMENTS WITH BACTERIA
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DOI：
10.1016/0003-2697(79)90569-4
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
TURNER, GL
中科院分区：
文献类型：
--
作者：
BERGERSEN, FJ;TURNER, GL
在无气相的实验中，氧化大豆豆血红蛋白和抹香鲸肌红蛋白被用作呼吸细菌的氧气源。这些携带 O2 的血红素蛋白提供分散的游离 O2，其浓度由其氧合和脱氧动力学常数定义，并且它们的光学吸收光谱表明游离 O2 的平均浓度。因此，它们可用于研究悬浮在其溶液中的细菌的游离氧浓度、呼吸速率和其他代谢过程之间的关系。描述了三种类型的装置以及根瘤菌呼吸和 N2 固定研究的例子。被给予。
Oxygenated soybean leghemoglobin and sperm whale myoglobin were used as sources of O2 for respiring bacteria in experiments with no gas phase. These O2-carrying hemoproteins provide dispersed free O2 at concentrations defined by the kinetic constants of their oxygenation and deoxygenation, and their optical absorption spectra indicate the average concentration of free O2. They can thus be used to study relationships between concentrations of free O2, rates of respiration and other metabolic processes in bacteria suspended in solutions of them. Three types of apparatus are described and examples of studies of respiration and N2 fixation by Rhizobium spp. are given.