SYSTEMIC AND LOCAL ANTIBODY-RESPONSES IN ELDERLY SUBJECTS GIVEN LIVE OR INACTIVATED INFLUENZA-A VIRUS-VACCINES
SYSTEMIC AND LOCAL ANTIBODY-RESPONSES IN ELDERLY SUBJECTS GIVEN LIVE OR INACTIVATED INFLUENZA-A VIRUS-VACCINES
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DOI：
10.1128/jcm.27.12.2666-2671.1989
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发表时间：
1989-12-01
影响因子：
9.4
通讯作者：
CLEMENTS, ML
中科院分区：
文献类型：
--
作者：
POWERS, DC;SEARS, SD;CLEMENTS, ML
本研究对80名流动老年人单独或联合给予鼻内减毒甲型流感/川崎/9/86 （H1N1）重组病毒和肠外灭活甲型流感/台湾/1/86 （H1N1）病毒。采用酶联免疫吸附法测定疫苗接种前和接种后1个月和3个月收集的血清和鼻洗标本中的血凝素特异性（HA）抗体。25名灭活病毒疫苗接种者血清免疫球蛋白G （IgG）和鼻洗液IgA HA反应分别在56%和20%，在27名两种疫苗接种者中分别为67%和48%，但在28名单独接种活病毒疫苗的疫苗接种者中分别只有36%和25%。单独给药或与活疫苗一起给药的灭活病毒诱导的血清抗体滴度高于单独给药的活病毒。相比之下，与单独接种疫苗相比，联合接种疫苗可更频繁、更大量地激发鼻腔IgA HA抗体。尽管存在这些差异，各组接种者的局部抗体峰值滴度是相似的。疫苗接种3个月后，灭活病毒疫苗接种者的血清IgG和鼻腔IgA HA抗体滴度分别高于疫苗接种前水平，灭活病毒疫苗接种者的血清IgG和鼻腔IgA HA抗体滴度分别高于50%和17%，两种疫苗接种者的血清IgG和鼻腔IgA HA抗体滴度分别高于46%和23%，而活病毒疫苗接种者的血清IgG和鼻腔IgA HA抗体滴度分别高于19%和7%。活甲型流感病毒诱导短暂的局部和全身抗体的发现，如果得到证实，表明活病毒疫苗接种可能不是老年人灭活病毒疫苗接种的合适替代或辅助。
Intranasal live attenuated cold-adapted (ca) influenza A/Kawasaki/9/86 (H1N1) reassortant virus and parenteral inactivated influenza A/Taiwan/1/86 (H1N1) virus were given alone or in combination to 80 ambulatory elderly subjects. An enzyme-linked immunosorbent assay was used to measure hemagglutinin-specific (HA) antibodies in serum and nasal wash specimens collected before vaccination and 1 and 3 months later. Serum immunoglobulin G (IgG) and nasal wash IgA HA responses were elicited in 56 and 20%, respectively, of 25 inactivated-virus vaccinees and in 67 and 48%, respectively, of 27 recipients of both vaccines but in only 36 and 25%, respectively, of 28 vaccinees given live virus alone. Inactivated virus, administered alone or with live vaccine, induced higher titers of serum antibody than did the live virus alone. In contrast, nasal IgA HA antibody was elicited more often and in greater quantity by the vaccine combination than by either vaccine alone. Despite these differences, the peak titers of local antibody mounted by each group of vaccinees were similar. By 3 months postvaccination, serum IgG and nasal IgA HA antibody titers remained elevated above prevaccination levels in 50 and 17%, respectively, of the inactivated-virus vaccinees and in 46 and 23%, respectively, of recipients of both vaccines but in only 19 and 7%, respectively, of the live-virus vaccinees. The finding that live ca influenza A virus induced short-lived local and systemic antibodies, if confirmed, suggests that live virus vaccination may not be a suitable alternative or adjunct to inactivated virus vaccination for the elderly.