Rapid sensing of antioxidant capacity based on electrochemiluminescence induced by electrochemically generated reactive oxygen species
Rapid sensing of antioxidant capacity based on electrochemiluminescence induced by electrochemically generated reactive oxygen species
复制标题
DOI：
10.1016/j.electacta.2016.11.012
复制
发表时间：
2016-12-20
影响因子：
6.6
通讯作者：
Tamiya, Eiichi
中科院分区：
文献类型：
--
作者：
Nagatani, Naoki;Inoue, Yuki;Tamiya, Eiichi
本文描述了一种使用带有丝网印刷电极的便携式电化学发光（ECL）装置测量抗氧化能力的简单快速的电化学发光方法。该ECL方法基于活性氧物质（ROS）、鲁米诺自由基和抗氧化剂的发光反应。ROS是通过溶解氧的电化学还原产生的，并与鲁米诺和抗氧化剂发生竞争反应。由于自由基被抗氧化剂中和，抑制了发光反应，所以ECL强度取决于抗氧化能力。通过ECL测量不同浓度已知量的特洛克斯（Trolox）绘制了抗氧化剂标准曲线，发现抗氧化能力的检测限为0.06 mM特洛克斯。通过与特洛克斯标准曲线比较，对22种饮料的抗氧化能力进行了评估。在丝网印刷电极上将样品溶液与鲁米诺混合后，ECL测量抗氧化能力所需时间仅为两分钟。（C）2016爱思唯尔有限公司。保留所有权利。
A simple and rapid electrochemiluminescence (ECL) method for the measurement of antioxidant capacity using a portable ECL device with a screen-printed electrode is described. The ECL method is based on the luminous reaction of reactive oxygen species (ROS), luminol radicals and antioxidants. ROS are generated by the electrochemical reduction of dissolved oxygen and competitively react with the luminol and antioxidants. The ECL intensity depends on the antioxidant capacity because the radicals are neutralized by the antioxidants, suppressing the luminous reaction. An antioxidant standard curve was generated using different concentrations of known amounts of Trolox by ECL measurement and the detection limit for antioxidant capacity was found to be 0.06 mM of Trolox. An antioxidant capacity of beverages (22 types) was evaluated by comparing with the standard curve of Trolox. The time necessary for the ECL measurement of antioxidant capacity is only two minutes after combining the sample solution with luminol on a screen-printed electrode. (C) 2016 Elsevier Ltd. All rights reserved.