Effect of secretory leucocyte protease inhibitor on early tendon-to-bone healing after anterior cruciate ligament reconstruction in a rat model.
Effect of secretory leucocyte protease inhibitor on early tendon-to-bone healing after anterior cruciate ligament reconstruction in a rat model.
复制标题
分泌型白细胞蛋白酶抑制剂对大鼠前交叉韧带重建术后早期腱-骨愈合的影响。
DOI：
10.1302/2046-3758.117.bjr-2021-0358.r2
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发表时间：
2022-07
影响因子：
4.6
通讯作者：
Zeng, C.
中科院分区：
文献类型：
--
作者：
Wu, Y.;Shao, Y.;Xie, D.;Pan, J.;Chen, H.;Yao, J.;Liang, J.;Ke, H.;Cai, D.;Zeng, C.
关键词：
验证分泌性白细胞蛋白酶抑制剂（SLPI）是否能促进前交叉韧带（ACL）重建后早期肌腱-骨愈合。体外实验：采用划痕法评价SLPI处理大鼠骨间充质干细胞（BMSCs）的移动性。通过实时定量PCR （real-time quantitative PCR, qPCR）分析成骨分化相关基因的表达水平，确定SLPI对骨髓间充质干细胞的成骨作用。体内：采用大鼠ACL重建模型验证SLPI对肌腱-骨愈合的影响。SLPI组和阴性对照（NC）组均予安乐死，进行组织学评估、显微ct扫描和生物力学测试。SLPI提高了BMSCs的迁移能力，上调了BMSCs成骨分化相关基因的表达。在体内，通过组织学评估，SLPI组在肌腱-骨界面处具有更高的组织学评分。显微ct显示SLPI组移植肌腱周围有更多的新骨形成和骨长入。肌腱-骨连接愈合强度评估显示，SLPI组具有更高的最大破坏力和刚度。SLPI可以通过促进骨髓间充质干细胞的迁移和成骨分化，有效促进前交叉韧带重建后肌腱到骨的早期愈合。本文引用本文：骨关节，2022;11(7):503-512。
To verify whether secretory leucocyte protease inhibitor (SLPI) can promote early tendon-to-bone healing after anterior cruciate ligament (ACL) reconstruction. In vitro: the mobility of the rat bone mesenchymal stem cells (BMSCs) treated with SLPI was evaluated by scratch assay. Then the expression levels of osteogenic differentiation-related genes were analyzed by real-time quantitative PCR (qPCR) to determine the osteogenic effect of SLPI on BMSCs. In vivo: a rat model of ACL reconstruction was used to verify the effect of SLPI on tendon-to-bone healing. All the animals of the SLPI group and the negative control (NC) group were euthanized for histological evaluation, micro-CT scanning, and biomechanical testing. SLPI improved the migration ability of BMSCs and upregulated the expression of genes related to osteogenic differentiation of BMSCs in vitro. In vivo, the SLPI group had higher histological scores at the tendon-bone interface by histological evaluation. Micro-CT showed more new bone formation and bone ingrowth around the grafted tendon in the SLPI group. Evaluation of the healing strength of the tendon-bone connection showed that the SLPI group had a higher maximum failure force and stiffness. SLPI can effectively promote early tendon-to-bone healing after ACL reconstruction via enhancing the migration and osteogenic differentiation of BMSCs. Cite this article: Bone Joint Res 2022;11(7):503–512.