Prospective of Genomics in Revealing Transmission, Reassortment and Evolution of Wildlife-Borne Avian Influenza A (H5N1) Viruses.
Prospective of Genomics in Revealing Transmission, Reassortment and Evolution of Wildlife-Borne Avian Influenza A (H5N1) Viruses.
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揭示野生动物传播的甲型禽流感 (H5N1) 病毒传播、重排和进化的基因组学前景。
DOI：
10.2174/138920211797904052
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发表时间：
2011-11
期刊：
影响因子：
2.6
通讯作者：
Shi W
中科院分区：
文献类型：
--
作者：
Lei F;Shi W
高致病性禽流感（HPAI）H5N1疾病的爆发已导致家禽和野生动物的重大损失以及60%的人类病死率。野鸟是所有禽流感病毒亚型的天然宿主，据报道，有120多种鸟类感染H5N1病毒。甲型流感病毒具有分段的RNA基因组，其特征在于频繁发生的遗传重配事件，这在病毒进化和新基因星座在免疫学上幼稚的人类和动物群体中的传播中起着非常重要的作用。全基因组或亚基因组序列的系统发育分析是描述遗传变异、新的重配事件和监测以追踪全球传播途径的标准手段。在本文中，特别强调了H5N1在野生动物种群之间的传播和循环，以及当H5N1病毒感染不同宿主（如鸟类、猪和人类）时与宿主间传播相关的重配事件。此外，我们还综述了H5N1病毒与其他亚型病毒（如H9N2和H6N1）之间编码内部蛋白的病毒片段的亚型间重配。最后，我们强调基因组序列在高致病性禽流感H5N1的分子流行病学分析中的有用性，以及现有分析方法的技术局限性，这些技术局限性阻碍了它们在病毒学研究中发挥更大的作用。
The outbreak of highly pathogenic avian influenza (HPAI) H5N1 disease has led to significant loss of poultry and wild life and case fatality rates in humans of 60%. Wild birds are natural hosts for all avian influenza virus subtypes and over120 bird species have been reported with evidence of H5N1 infection. Influenza A viruses possess a segmented RNA genome and are characterized by frequently occurring genetic reassortment events, which play a very important role in virus evolution and the spread of novel gene constellations in immunologically naïve human and animal populations. Phylogenetic analysis of whole genome or sub-genomic sequences is a standard means for delineating genetic variation, novel reassortment events, and surveillance to trace the global transmission pathways. In this paper, special emphasis is given to the transmission and circulation of H5N1 among wild life populations, and to the reassortment events that are associated with inter-host transmission of the H5N1 viruses when they infect different hosts, such as birds, pigs and humans. In addition, we review the inter-subtype reassortment of the viral segments encoding inner proteins between the H5N1 viruses and viruses of other subtypes, such as H9N2 and H6N1. Finally, we highlight the usefulness of genomic sequences in molecular epidemiological analysis of HPAI H5N1 and the technical limitations in existing analytical methods that hinder them from playing a greater role in virological research.