Enantiomeric resolution using the macrocyclic antibiotics rifamycin B and rifamycin SV as chiral selectors for capillary electrophoresis.
Enantiomeric resolution using the macrocyclic antibiotics rifamycin B and rifamycin SV as chiral selectors for capillary electrophoresis.
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DOI：
10.1016/0021-9673(95)00608-p
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发表时间：
1995-11
期刊：
影响因子：
--
通讯作者：
T. Ward;C. Dann;A. Blaylock
中科院分区：
文献类型：
--
作者：
T. Ward;C. Dann;A. Blaylock
利福霉素B和利福霉素SV属于称为安莎霉素的大环抗生素类。这些大环抗生素作为手性选择剂在毛细管电泳中对许多手性化合物进行了对映选择性拆分。它们含有能够提供实现对映异构体之间手性识别所需的多种相互作用类型的基团。事实上，它们似乎在它们可以对映体拆分的化合物类型方面是互补的。利福霉素B显示对带正电荷的化合物具有对映选择性，而利福霉素SV对带负电荷的溶质具有对映选择性。用于检测的波长的选择显著影响灵敏度。监测与手性选择剂的吸收最小值一致的波长之一增强了灵敏度。通过保持柱上注射的分析物的量尽可能低来提高分离度，并且证明使用间接检测在另一对映异构体存在下可以检测到低至0.1%的一种对映异构体。
Rifamycin B and rifamycin SV belong to the class of macrocyclic antibiotics known as ansamycins. These macrocyclic antibiotics were used as chiral selectors in capillary electrophoresis to enantioselectively resolve a number of chiral compounds. They contain groups capable of providing the types of multiple interactions necessary to achieve chiral recognition between enantiomers. In fact, they appear to be complimentary in the types of compounds they can enantiomerically resolve. Rifamycin B is shown to be enantioselective towards positively charged compounds, while rifamycin SV was enantioselective towards negatively charged solutes. The choice of wavelength for detection significantly affects sensitivity. Monitoring one of the wavelengths which coincide with the absorption minima of the chiral selector enhances sensitivity. Resolution is enhanced by keeping the amount of analyte injected on column as low as possible and it is demonstrated that it is possible to detect as little as 0.1% of one enantiomer in the presence of the other enantiomer using indirect detection.