Mouse plasmacytoid dendritic cells derive exclusively from estrogen-resistant myeloid progenitors
Mouse plasmacytoid dendritic cells derive exclusively from estrogen-resistant myeloid progenitors
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DOI：
10.1182/blood-2005-11-4545
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发表时间：
2006-08-01
期刊：
影响因子：
20.3
通讯作者：
Allman, David
中科院分区：
文献类型：
--
作者：
Harman, Benjamin C.;Miller, Juli P.;Allman, David
目前的模型预测，小鼠浆细胞样树突状细胞（PDCs）来自淋巴祖细胞。然而，我们发现PDCs完全来自普通髓系祖细胞（CMPs），其特征是几个淋巴相关基因的低水平表达，包括RAG 2/GFP报告基因转基因。这一结论得到了过继转移实验和雌激素治疗策略的支持，雌激素治疗策略导致非常早期的淋巴祖细胞显著耗竭，而不影响RAG 2/GFP（+）CMP或发育动力学、RAG介导的重组酶活性和PDC的细胞因子产生。这些数据表明，IPDCs只产生于早期髓系祖细胞和混杂的低水平表达的淋巴相关基因是一个共同的特点PDC祖细胞之间的CMP。
Current models predict that mouse plasmacytoid dendritic cells (PDCs) derive from lymphoid progenitors. However, we show PDCs arise exclusively from common myeloid progenitors (CMPs) characterized by low-level expression of several lymphoid-associated genes, including a RAG2/GFP reporter transgene. This conclusion is supported by both adoptive transfer experiments and an estrogen treatment strategy that led to marked depletion of very early lymphoid progenitors without affecting RAG2/GFP(+) CMPs or the developmental kinetics, RAG-mediated recombinase activity, and cytokine production of PDCs. These data suggest that IPDCs arise exclusively from early myeloid progenitors and that promiscuous low-level expression of lymphoidassociated genes is a general feature of PDC progenitors among CMPs.