Lipid production through the single-step microwave hydrolysis of macroalgae using the oleaginous yeast Metschnikowia pulcherrima
Lipid production through the single-step microwave hydrolysis of macroalgae using the oleaginous yeast Metschnikowia pulcherrima
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DOI：
10.1016/j.algal.2019.101411
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发表时间：
2019-03-01
影响因子：
5.1
通讯作者：
Chuck, Christopher J.
中科院分区：
文献类型：
--
作者：
Abeln, Felix;Fan, Jiajun;Chuck, Christopher J.
大型藻类（海藻）是一种新兴的食品资源，也是商品和特种化学品的生产资源。在这项研究中，一个单步微波过程被用来解聚一系列的大型藻类原产于英国，生产适合微生物发酵的生长培养基。培养基中含有一系列的单-和多糖以及宏-和微量营养素，可以代谢的产油酵母Metschnikowia pulcherrima。在12种大型海藻中，褐藻的发酵潜力最高，尤其是海带。应用十个本地M的投资组合。检测了Pulcherrima菌株、酵母生长动力学以及脂质和2-苯基乙醇的生产，生产率和生长速率依赖于菌株。在2L规模上，通过利用来自S. latissima，没有额外的酶。此外，酵母降解了一系列的发酵抑制剂后，在高温和长时间的微波处理释放。由于大型藻类可以培养到食品级，该系统提供了一种新的，潜在的低成本的途径，以食用微生物油以及可再生的油脂化学品的原料。
Macroalgae (seaweeds) represent an emerging resource for food and the production of commodity and specialty chemicals. In this study, a single-step microwave process was used to depolymerise a range of macroalgae native to the United Kingdom, producing a growth medium suitable for microbial fermentation. The medium contained a range of mono- and polysaccharides as well as macro- and micronutrients that could be metabolised by the oleaginous yeast Metschnikowia pulcherrima. Among twelve macroalgae species, the brown seaweeds exhibited the highest fermentation potential, especially the kelp Saccharina latissima. Applying a portfolio of ten native M. pulcherrima strains, yeast growth kinetics, as well as production of lipids and 2-phenylethanol were examined, with productivity and growth rate being strain dependent. On the 2 L scale, 6.9 g L-1 yeast biomass - a yield of 0.14 g g(-1) with respect to the supplied macroalgae - containing 37.2% (w/w) lipid was achieved through utilisation of the proteins, mono- and polysaccharides from S. latissima, with no additional enzymes. In addition, the yeast degraded a range of fermentation inhibitors released upon microwave processing at high temperatures and long holding times. As macroalgae can be cultured to food grade, this system offers a novel, potentially low-cost route to edible microbial oils as well as a renewable feedstock for oleochemicals.