Integrated dielectrophoretic and impedimetric biosensor provides a template for universal biomarker sensing in clinical samples
Integrated dielectrophoretic and impedimetric biosensor provides a template for universal biomarker sensing in clinical samples
复制标题
集成介电泳和阻抗生物传感器为临床样品中的通用生物标志物传感提供了模板
DOI：
10.1002/elps.202000347
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发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Nawarathna, Dharmakeerthi
中科院分区：
文献类型：
--
作者：
Velmanickam, Logeeshan;Jayasooriya, Vidura;Nawarathna, Dharmakeerthi
体液中核酸和蛋白质组生物标志物的检测和定量是许多医学筛查和诊断的关键部分。然而，目前的大多数检测平台并不适合在医疗保健场所进行常规、快速和低成本的检测。为了解决这个问题，我们开发了一种一次性通用护理点生物传感器的概念，可以检测和定量稀释血清样本中的核酸和蛋白质组生物标记物。传感的中心原则是利用介电、电热效应和热电泳法选择性地、快速地分离电极中感兴趣的生物标志物，然后使用电阻抗进行定量。将该传感器应用于血清稀释样品中微量RNA和抗原生物标记物分子的直接定量，检测灵敏度为1012~1015Ω/M，检测灵敏度为1012~1015 DNA/M，检测时间为30min，每次检测成本低于50美元。
The detection and quantification of nucleic acid and proteomic biomarkers in bodily fluids is a critical part of many medical screening and diagnoses. However, majority of the current detection platforms are not ideal for routine, rapid, and low‐cost testing in point‐of‐care settings. To address this issue, we developed a concept for a disposable universal point‐of‐care biosensor that can detect and quantify nucleic acid and proteomic biomarkers in diluted serum samples. The central tenet of sensing is the use of dielectrophoresis, electrothermal effects, and thermophoresis to selectively and rapidly isolate the biomarkers of interest in electrodes and then quantify using electrical impedance. When the sensor was applied to quantify microRNA and antigen biomarker molecules directly in diluted serum samples, it produced a LOD values in the fM range and sensitivity values from 1012to 1015Ω/M with a 30 min assay time and assay cost of less than $50 per assay.