T-cell subpopulations in lesions of oral lichen planus.
T-cell subpopulations in lesions of oral lichen planus.
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发表时间：
2013
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通讯作者：
M. Popovska;L. Grchevska;V. Popovski;A. Atanasovska-Stojanovska;A. Minovska;Z. Belazelkoska;V. Radojkova-Nikolovska;Kristina Mitik;S. Strezovska
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作者：
M. Popovska;L. Grchevska;V. Popovski;A. Atanasovska-Stojanovska;A. Minovska;Z. Belazelkoska;V. Radojkova-Nikolovska;Kristina Mitik;S. Strezovska
目的探讨T（CD 3）细胞及其亚群在糜烂性扁平苔藓（EOLP）组织中的分布和频率。材料与方法颊粘膜组织标本取自14名健康人-对照组（CG）-和17名EF-OLP受试者。应用单克隆抗体、T（CD_3）细胞、T细胞亚群、CD_4、CD_8及CD_4/CD_8比值测定。对两组的上皮和固有层细胞进行定量和定性测定。使用Student t检验分析数据。结果对照组T（CD 3）细胞为5.95 ± 2.12%，实验组为9.80 ± 4.04%，两组比较有显著性差异（P<0.001）。T（CD_3）细胞在固有层中的分布为25.35 ± 12.04%，与对照组比较有显著性差异（P<0.001）。对照组CD_4上皮细胞为3.45 ± 2.05%，实验组为4.00 ± 1.95%（P<0.4）。对照组CD_8细胞为2.50 ± 1.8%，实验组为5.80 ± 3.72%（P<0.001）。CD_4/CD_8比值为0.51 ± 0.12%，与对照组比较明显降低。CD 4细胞（10.30 ± 7.60%）和CD 8细胞（15.05 ± 5.20%）在固有层中的分布明显高于上皮细胞（P<0.001）。上皮细胞中CD_4/CD_8比值为0.51 ± 0.12%，固有层细胞中CD_4/CD_8比值为0.68 ± 0.48%，两者间差异无统计学意义（P> 0.05）。结论正常对照组与实验组T淋巴细胞亚群分布有显著性差异（P<0.001）。观察到固有层中CD 4和CD 8细胞的分布和频率增加。这些主要位于乳头状层的亚基底区域，很少见于上皮内区域。
AIM OF THE STUDY To determine the distribution and frequency of T(CD3) cells and cell sub-populations in tissue specimens of erosive lichen planus (EOLP). MATERIAL AND METHOD Tissue specimens from buccal mucosa were taken from 14 healthy individuals--control group (CG)--and 17 subjects with EF-OLP. Applying monoclonal antibodies, T(CD3) cells, T cell subpopulations, The CD4, CD8 and CD4/CD8 ratios in both groups were determined. Cells in the epithelium and lamina propria were quantitatively and qualitatively determined in both groups. Data were analysed using the Student's t-test. RESULTS There were 5.95 2.12% T(CD3) cells in the control group as against 9.80 4.04% in the examined group (p<0.001). The distribution of T(CD3) cells in the lamina propria was 25.35 12.04% in the examined group compared to the control group (p<0.001). There were 3.45 2.05% CD4 epithelial cells in the control group and 4.00 1.95% in the examined group (p<0.4). There were 2.50 1.8% CD8 cells in the control group and 5.80 3.72% in the examined group (p<0.001). The CD4/CD8 ratio was 0.51 0.12% in the examined group and it was evidently reduced in comparison with the control group. An increased distribution of CD4 cells (10.30 7.60%) and CD8 cells (15.05 5.20%) in the lamina propria compared with the epithelium was observed in the examined group (p<0.001). The CD4/CD8 ratio in the epithelium was 0.51 0.12% as against the ratio of the lamina propria, which was slightly increased (0.68 0.48%) with a low statistically significant difference (p<0.05). CONCLUSION Differences in the distribution of T-lymphocyte subsets between the control and examined groups were found (p<0.001). An increased distribution and frequency of CD4 and CD8 cells in the lamina propria was observed. These were predominantly located in the sub-basal region of the stratum papillare and rarely seen in the intra-epithelial region.