Bone Morphogenetic Protein-2 Release from Composite Hydrogels of Oligo(poly(ethylene glycol) fumarate) and Gelatin
Bone Morphogenetic Protein-2 Release from Composite Hydrogels of Oligo(poly(ethylene glycol) fumarate) and Gelatin
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DOI：
10.1007/s11095-013-1077-5
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发表时间：
2013-09-01
影响因子：
3.7
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Kinard, Lucas A.;Chu, Chia-Ye;Mikos, Antonios G.
以低聚富马酸乙二醇酯（OPF）和明胶微粒（GM）为载体，通过改变OPF体相中明胶的含量和种类（酸性和碱性），制备了具有不同物理特性的复合水凝胶，并将其作为骨形态发生蛋白-2（BMP-2）的载体应用于骨组织工程。复合材料具有不同的物理性能（降解，溶胀，和机械性能）和不同的BMP-2加载阶段进行了研究，以确定这些因素对BMP-2释放超过28 days.Overall，更高的明胶量增加的降解和溶胀的复合材料，和酸性GM进一步增加的降解和溶胀，并降低复合材料的压缩模量的影响。影响BMP-2从复合材料中释放的最重要因素是生长因子的加载阶段：GM负荷减少了突释，增加了实验后期BMP-2的释放，并且在降解较快的样品中增加了累积释放。结果表明，物理性质和BMP-水凝胶复合材料的释放动力学可以通过在水凝胶复合材料制造时调节多个参数来控制。
Hydrogel composites of oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles (GMs) were investigated as carriers of bone morphogenetic protein-2 (BMP-2) for bone tissue engineering applications.Hydrogel composites with different physical characteristics were prepared by changing the amount and type (acidic vs. basic) of gelatin incorporated in the OPF bulk phase. Composites with differing physical properties (degradation, swelling, and mechanical properties) and differing BMP-2 loading phase were investigated to determine the effect of these factors on BMP-2 release profiles over 28 days.Overall, higher gelatin amount increased the degradation and swelling of composites, and acidic GMs further increased the degradation and swelling and reduced the compressive modulus of the composites. The most significant factor affecting the release of BMP-2 from composites was the loading phase of the growth factor: GM loading reduced the burst release, increased BMP-2 release during the later phases of the experiment, and increased the cumulative release in faster degrading samples.The results indicate that the physical properties and the BMP-2 release kinetics of hydrogel composites can be controlled by adjusting multiple parameters at the time of the hydrogel composite fabrication.