PATTERN OF INJURY AND THE ROLE OF NEUTROPHILS IN REPERFUSION INJURY OF RAT LUNG
PATTERN OF INJURY AND THE ROLE OF NEUTROPHILS IN REPERFUSION INJURY OF RAT LUNG
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DOI：
10.1006/jsre.1995.1112
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发表时间：
1995-06-01
影响因子：
2.2
通讯作者：
WARD, PA
中科院分区：
文献类型：
--
作者：
EPPINGER, MJ;JONES, ML;WARD, PA
使用大鼠肺模型，我们试图表征缺血-再灌注损伤的时间过程和中性粒细胞在损伤发展中的作用。成年雄性Long-Evans大鼠进行左胸廓切开术，解剖并夹闭左肺动脉、支气管和静脉90分钟，导致完全左肺缺血。然后将肺通气并再灌注长达4小时，时间匹配的假手术动物进行相同的开胸术和肺门解剖，但肺没有缺血。使用I-125标记的牛血清白蛋白的血管渗透性作为再灌注损伤的量度，观察到损伤的双峰模式。与假手术对照相比，进行缺血-再灌注的动物在再灌注30分钟时表现出显著的早期肺损伤再灌注4 h后，肺损伤出现第二个高峰（P < 0.0001）（P < 0.001），再灌注肺组织中的髓过氧化物酶活性（中性粒细胞隔离的量度）在再灌注时间过程中增加，为了确定中性粒细胞在肺再灌注损伤发展中的作用，在手术前一天，接受相同缺血-再灌注方案的另外的动物接受兔抗大鼠中性粒细胞血清或免疫前血清，
Using a rat lung model, we sought to characterize the time course for ischemia-reperfusion injury and the role of neutrophils in the development of injury, Adult male Long-Evans rats underwent left thoracotomy with dissection and clamping of the left pulmonary artery, bronchus, and vein for 90 min, resulting in complete left lung ischemia, The lungs were then ventilated and reperfused for up to 4 hr, Time-matched sham animals underwent the identical thoracotomy and hilar dissection, but the lungs were not rendered ischemic, Using vascular permeability of I-125-labeled bovine serum albumin as a measure of reperfusion injury, a bimodal pattern of injury was observed, Compared to sham controls, animals undergoing ischemia-reperfusion demonstrated a significant early phase of lung injury at 30 min of reperfusion (P < 0.0001), followed by partial recovery, A second peak of lung injury was noted after 4 hr of reperfusion (P < 0.001), Myeloperoxidase activity in reperfused lung tissue, a measure of neutrophil sequestration, increased during the reperfusion time course, To determine the role of neutrophils in the development of lung reperfusion injury, additional animals undergoing the identical ischemia-reperfusion protocol received either rabbit anti-rat neutrophil serum or preimmune serum the day prior to operation, Profound neutropenia (