Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development
Myc-dependent regulation of ribosomal RNA synthesis during Drosophila development
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DOI：
10.1038/ncb1223
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发表时间：
2005-03-01
影响因子：
21.3
通讯作者：
Edgar, BA
中科院分区：
文献类型：
--
作者：
Grewal, SS;Li, L;Edgar, BA
核糖体数量的调控被认为可控制细胞生长（1）。核糖体RNA（rRNA）的合成是核糖体生物发生的一个限速步骤，并且rRNA的合成速率通常根据细胞的生长状态而改变（2,3）。尽管对单细胞系统的研究已经阐述了这种情况发生的机制（4,5），但很少有研究采用遗传学方法来检测后生动物中rRNA合成的生长依赖性调控。在此，我们表明在黑腹果蝇中，Myc（dMyc）是rRNA合成的一个调节因子。在幼虫发育过程中，dMyc的表达对于控制rRNA合成和核糖体生物发生既是必需的也是充分的。dMyc对rRNA合成的刺激是通过RNA聚合酶I转录机制水平的快速、协同增加来介导的。此外，dMyc在幼虫翅成虫盘中的生长效应需要从头合成rRNA。我们认为，在动物发育过程中，对rRNA合成和核糖体生物发生的控制是Myc的一个基本功能。
Regulating ribosome number is thought to control cellular growth(1). Synthesis of ribosomal RNA ( rRNA) is a limiting step in ribosome biogenesis and rates of rRNA synthesis are generally altered depending on the growth status of a cell(2,3). Although studies in unicellular systems have addressed the mechanisms by which this occurs(4,5), few studies have applied a genetic approach to examine growth-dependent control of rRNA synthesis in metazoans. Here, we show that in Drosophila melanogaster Myc ( dMyc) is a regulator of rRNA synthesis. Expression of dMyc is both necessary and sufficient to control rRNA synthesis and ribosome biogenesis during larval development. Stimulation of rRNA synthesis by dMyc is mediated through a rapid, coordinated increase in the levels of the Pol I transcriptional machinery. In addition, the growth effects of dMyc in larval wing imaginal discs require de novo rRNA synthesis. We suggest that during animal development, the control of rRNA synthesis and ribosome biogenesis is an essential Myc function.